Water use strategies and drought intensity define the relative contributions of hydraulic failure and carbohydrate depletion during seedling mortality

• Published in: Plant physiology and biochemistry 2020-08, Vol.153, p.106-118
• Main Authors: Li, Qiang, Zhao, Mingming, Wang, Ning, Liu, Shuna, Wang, Jingwen, Zhang, Wenxin, Yang, Ning, Fan, Peixian, Wang, Renqing, Wang, Hui, Du, Ning
• Format: Article
• Language: English
• Subjects: Anisohydric; Carbon starvation; Hydraulic failure; Intense drought; Isohydric; Mild drought
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2020.05.023

and carbon-related measurements can help elucidate drought-induced plant mortality. To study drought mortality mechanisms, seedlings of two woody species, including the anisohydric Robinia pseudoacacia and isohydric Quercus acutissima, were cultivated in a greenhouse and subjected to intense drought by withholding water and mild drought by adding half of the amount of daily water lost. Patterns of leaf and root gas exchange, leaf surface areas, growth, leaf and stem hydraulics, and carbohydrate dynamics were determined in drought-stressed and control seedlings. We detected a complete loss of hydraulic conductivity and partial depletion of total nonstructural carbohydrates contents (TNC) in the dead seedlings. We also found that intense drought triggered a more rapid decrease in plant water potential and a faster drop in net photosynthesis below zero, and a greater TNC loss in dead seedlings than mild drought. Additionally, anisohydric R. pseudoacacia suffered a rapider death than the isohydric Q. acutissima. Based on these findings, we propose that hydraulic conductivity loss and carbon limitation jointly contributed to drought-induced death, while the relative contributions could be altered by drought intensity. We thus believe that it is important to illustrate the mechanistic relationships between stress intensity and carbon-hydraulics coupling in the context of isohydric vs. anisohydric hydraulic strategies. •There were complete conductivity loss and partial C depletion in dead plants.•Intense drought also resulted in a smaller C loss and in a rapid conductivity loss when compared with mild drought.•Anisohydric R. pseudoacacia suffered higher risk of hydraulic failure, and a rapid mortality than isohydric Q. acutissima.