Bioconversion of arachidonic acid into human 14,15-hepoxilin B3 and 13,14,15-trioxilin B3 by recombinant cells expressing microbial 15-lipoxygenase without and with epoxide hydrolase

• Published in: Biotechnology letters 2020-10, Vol.42 (10), p.2001-2009
• Main Authors: Lee, J., Kim, T.-H., Oh, D.-K.
• Format: Article
• Language: English
• Subjects: Acids; Applied Microbiology; Arachidonic acid; Bacteria; Biochemistry; Bioconversion; Biomedical and Life Sciences; Biotechnology; E coli; Epoxide hydrolase; Life Sciences; Lipoxygenase; Liquid oxygen; Microbiology; Microorganisms; Original Research Paper
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-020-02932-y

Objective To produce high concentrations of 13-hydroxy-14,15-epoxy-eicosatrienoic acid (14,15-hepoxilin B 3 , 14,15-HXB 3 ) and 13,14,15-trihydroxy-eicosatrienoic acid (13,14,15-trioxilin B 3 , 13,14,15-TrXB 3 ) from arachidonic acid (ARA) using microbial 15-lipoxygenase (15-LOX) without and with epoxide hydrolase (EH), respectively. Results The products obtained from the bioconversion of ARA by recombinant Escherichia coli cells containing Archangium violaceum 15-LOX without and with Myxococcus xanthus EH were identified as 14,15-HXB 3 and 13,14,15-TrXB 3 , respectively. Under the optimal conditions of 30 g cells L −1 , 200 mM ARA, 25 °C, and initial pH 7.5, the cells converted 200 mM ARA into 192 mM 14,15-HXB 3 and 100 mM 13,14,15-TrXB 3 for 150 min, with conversion yields of 96 and 51% and productivities of 77 and 40 mM h −1 , respectively. Conclusion These are the highest concentrations, productivities, and yields of hepoxilin and trioxilin from ARA reported thus far.