Mechanisms of programmed cell death in the midgut and salivary glands from Bradysia hygida (Diptera: Sciaridae) during pupal–adult metamorphosis

Programmed cell death is involved with the degeneration/remodeling of larval tissues and organs during holometabolous development. The midgut is a model to study the types of programmed cell death associated with metamorphosis because its structure while degenerating is a substrate for the formation...

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Bibliographic Details
Published in:Cell biology international 2020-10, Vol.44 (10), p.1981-1990
Main Authors: Cassia Santos Przepiura, Thaylise, Navarro, Aryelle M., Rosa Ribeiro, Rafaela, Gomes, José R., Pitthan, Karina V., Miranda Soares, Maria A.
Format: Article
Language:English
Subjects:
Acridine orange
Actin
Apoptosis
autophagy
Bradysia
Bradysia hygida
Cell culture
Cell death
Cytoskeleton
Degeneration
development
Glycogen
Histolysis
Localization
Metamorphosis
Midgut
Salivary gland
salivary glands
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Summary:Programmed cell death is involved with the degeneration/remodeling of larval tissues and organs during holometabolous development. The midgut is a model to study the types of programmed cell death associated with metamorphosis because its structure while degenerating is a substrate for the formation of the adult organ. Another model is the salivary glands from dipteran because their elimination involves different cell death modes. This study aimed to investigate the models of programmed cell death operating during midgut replacement and salivary gland histolysis in Bradysia hygida. We carried out experiments of real‐time observations, morphological analysis, glycogen detection, filamentous‐actin localization, and nuclear acridine orange staining. Our findings allow us to establish that an intact actin cytoskeleton is required for midgut replacement in B. hygida and nuclear condensation and acridine orange staining precede the death of the larval cells. Salivary glands in histolysis present cytoplasmic blebbing, nuclear retraction, and acridine orange staining. This process can be partially reproduced in vitro. We propose that the larval midgut death involves autophagic and apoptotic features and apoptosis is a mechanism involved with salivary gland histolysis.
ISSN:1065-6995
1095-8355
DOI:10.1002/cbin.11404