Contribution of immunophenotype to the investigation and differential diagnosis of Burkitt lymphoma, double‐hit high‐grade B‐cell lymphoma, and single‐hit MYC‐rearranged diffuse large B‐cell lymphoma

Background There are no immunophenotypic guidelines for the investigation of MYC‐rearranged lymphomas. We aimed to identify simple immunophenotypic features that would help to differentiate between MYC‐rearranged lymphomas and guide cytogenetic analysis. Methods We reviewed diagnostic samples from p...

Full description

Saved in:
Bibliographic Details
Published in:Cytometry. Part B, Clinical cytometry Clinical cytometry, 2020-09, Vol.98 (5), p.412-420
Main Authors: Tsagarakis, Nikolaos J., Papadhimitriou, Stefanos I., Pavlidis, Dimitris, Liapis, Konstantinos, Gortzolidis, Georgios, Kostopoulos, Ioannis V., Marinakis, Theodoros, Paterakis, Georgios
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Bcl-6 protein
Burkitt lymphoma
Burkitt's lymphoma
CD20 antigen
CD22 antigen
CD23 antigen
CD27 antigen
CD38 antigen
CD43 antigen
Cytogenetics
Diagnosis
Diagnostic systems
Differential diagnosis
DLBCL
double‐hit lymphoma
Flow cytometry
Fluorescence
Fluorescence in situ hybridization
immunophenotype
Lymphoma
Myc protein
MYC rearrangement
Scattering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background There are no immunophenotypic guidelines for the investigation of MYC‐rearranged lymphomas. We aimed to identify simple immunophenotypic features that would help to differentiate between MYC‐rearranged lymphomas and guide cytogenetic analysis. Methods We reviewed diagnostic samples from patients diagnosed with Burkitt lymphoma (BL), double‐hit lymphoma (DHL), MYC‐rearranged diffuse large B‐cell lymphoma (MYC‐DLBCL), and standard (non‐MYC‐rearranged) DLBCL over the last decade in our Institution. Using flow cytometry (with antibodies CD20, CD10, CD38, bcl‐2, Ki‐67, FMC‐7, CD43, CD27, CD79b, CD23, and CD22) we determined antigen% expression and median‐fluorescence intensity ratios (MFIR). The forward scatter (FS) and side scatter (SS) characteristics of tumor B‐cells were compared with normal T‐cells (B/T ratios) for patients with MYC‐rearranged lymphomas. Results We identified 51 patients of whom 14 had BL, 10 had DHL (6 MYC+/BCL2+; 4 MYC+/BCL6+), 8 MYC‐DLBCL, and 19 standard DLBCL. The significant differences (p  90, CD10% > 80, CD10MFIR > 10, bcl‐2%  70 was characteristic of BL. “Deviation” from these cut‐offs should raise suspicion for DHL and, therefore, BCL2 and/or BCL6 FISH is required. We also found that a diagnosis of DHL rather than of MYC‐DLBCL was significantly associated with CD10% > 60, Ki‐67% > 50, and SS (B/T)
ISSN:1552-4949
1552-4957
DOI:10.1002/cyto.b.21887