Luteimonas granuli sp. nov., Isolated from Granules of the Wastewater Treatment Plant

• Published in: Current microbiology 2020-09, Vol.77 (9), p.2002-2007
• Main Authors: Siddiqi, Muhammad Zubair, Yeon, Jun Mo, Choi, Hanna, Lee, Jae Hag, Kim, Sang Yong, Wee, Ji-Hyang, Im, Wan Taek
• Format: Article
• Language: English
• Subjects: Bacterial Typing Techniques; Biomedical and Life Sciences; Biotechnology; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty Acids; Gene sequencing; Genomes; Granular materials; Life Sciences; Low level; Microbiology; New species; Nucleic Acid Hybridization; Phospholipids; Phylogeny; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Ubiquinone; Wastewater treatment; Wastewater treatment plants; Water Purification; Water treatment; Xanthomonadaceae - genetics
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-020-02066-4

A Gram-reaction negative, aerobic, non-motile, light yellow colored, and rod-shaped bacterium (designated Gr-4 T ) isolated from granules of a wastewater treatment plant, was characterized by a polyphasic approach to clarify its taxonomic position. Strain Gr-4 T was observed to grew optimally at 30 ºC and at pH 7.0 on R2A medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Gr-4 T belongs to the genus Luteimonas of the family Xanthomonadaceae and was most closely related to Luteimonas padinae CDR SL 15 T (99.1%), Luteimonas terricola DSM 22344 T (98.5%) and Luteimonas arsenica 26-35 T (97.6). The genome comprises 2,917,404 bp with a G+C content of 70.5 mol%. The ANI value between strain Gr-4 T and Luteimonas padinae CDR SL 15 T was 87.3%. The DNA–DNA relatedness value between strain Gr-4 T and Luteimonas padinae CDR SL 15 T , Luteimonas terricola DSM 22344 T was 36.4 ± 1.3% and 14.2 ± 1.7%, respectively. The predominant quinone was Q-8. The major fatty acids were iso-C 15:0 , iso-C 16:0 and summed feature 9 (comprising iso-C 17:1 ω 9 c and/or C 16:0 10-methyl) supported the affiliation of strain Gr-4 T to the genus Luteimonas . Moreover, the physiological, biochemical results, and low level of ANI and DNA–DNA relatedness value allowed the phenotypic and genotypic differentiation of strains Gr-4 T from other Luteimonas species with validly published names. The novel isolate therefore represents a novel species, for which the name Luteimonas granuli sp. nov. is proposed, with the type strain Gr-4 T (=KACC 16614 T  = JCM 18203 T ).