Polymorphism in the 3′-UTR of LIF but Not in the ATF6B Gene Associates with Schizophrenia Susceptibility: a Case-Control Study and In Silico Analyses

• Published in: Journal of molecular neuroscience 2020-12, Vol.70 (12), p.2093-2101
• Main Authors: Moudi, Mahdiyeh, Sargazi, Saman, Heidari Nia, Milad, Saravani, Ramin, Shirvaliloo, Milad, Shakiba, Mansoor
• Format: Article
• Language: English
• Subjects: 3' Untranslated regions; Binding sites; Biomedical and Life Sciences; Biomedicine; Cell Biology; Folding structures; Gene polymorphism; Genetic factors; Genotyping; Mental disorders; MicroRNAs; miRNA; mRNA; Neurochemistry; Neurology; Neurosciences; Nucleotides; Polymerase chain reaction; Polymorphism; Proteomics; Restriction fragment length polymorphism; Schizophrenia; Single-nucleotide polymorphism
• ISSN: 0895-8696; 1559-1166
• DOI: 10.1007/s12031-020-01616-6

Schizophrenia (SCZ) is a multifactorial disorder caused by environmental and genetic factors. Studies have shown that various single-nucleotide polymorphisms (SNPs) in the binding sites of microRNAs contribute to the risk of developing SCZ. We aimed to investigate whether the variants located in the 3′-UTR region of LIF (rs929271T>G) and ATF6B (rs8283G>A) were associated with increased susceptibility to SCZ in a population from the south-east of Iran. In this case-control study, a total of 396 subjects were recruited. SNPs were genotyped via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Genotyping results showed that the G allele of rs929271 significantly increased the risk of SCZ (OR = 1.58 95%CI = 1.19–2.10, p  = 0.001). As for rs929271, the GG genotype of co-dominant (OR = 2.54 95%CI = 1.39–4.64, p  = 0.002) and recessive (OR = 2.91 95%CI = 1.77–4.80, p