Simultaneous analysis of retinol, six carotenoids, two tocopherols, and coenzyme Q10 from human plasma by HPLC

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2020-08, Vol.1151, p.122158-122158, Article 122158
• Main Authors: Boulet, Lysiane, Alex, Brigitte, Clavey, Nadège, Martinez, Julie, Ducros, Véronique
• Format: Article
• Language: English
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2020.122158

•A single HPLC method for major plasma lipophilic antioxidants, including coenzyme Q10.•Analytical performance allowing their determination at physiological levels.•Suitable for the determination of these compounds in large-scale population follow-up. Lipophilic antioxidant determination is of relevance in health and diseases. Several HPLC methods exists but rare are those including coenzyme Q10 with carotenoids, retinol and tocopherols. Here a single-step extraction was proposed for the detection of retinol, α and γ-tocopherols, lutein, zeaxanthin, trans-ß-carotene, α-carotene, ß-cryptoxanthin and lycopene as well as coenzyme Q10. A single HPLC column was used and UV–vis diode array detection was performed. Echinenone, alpha-tocopherol nicotinate and coenzyme Q4 were employed as internal standards. Intra-assay and inter-assay precision were respectively 1.4–7.9% and 2.2–15.8%. Accuracy was validated using SRM 968e. LOD (limit of detection) and LOQ (limit of quantification) obtained were sufficient for nutritional epidemiological study and routine clinical application.