Doping Polysulfone Membrane with Alpha‐Tocopherol and Alpha‐Lipoic Acid for Suppressing Oxidative Stress Induced by Hemodialysis Treatment

• Published in: Macromolecular bioscience 2020-07, Vol.20 (7), p.e2000046-n/a
• Main Authors: Kohlová, Michaela, Rocha, Susana, Gomes Amorim, Célia, Nova Araújo, Alberto, Santos‐Silva, Alice, Solich, Petr, Branco da Silva Montenegro, Maria Conceição
• Format: Article
• Language: English
• Subjects: alpha‐lipoic acid; alpha‐tocopherol; Antioxidants; Biocompatibility; Blood plasma; Cardiovascular diseases; Complement activation; Free radicals; Hemodialysis; Lipid peroxidation; Lipids; Lipoic acid; Membranes; Oxidative stress; Peroxidation; Polysulfone; polysulfone membranes; Polysulfone resins; Protein adsorption; Spin coating; Tocopherol; Vitamin E
• ISSN: 1616-5187; 1616-5195
• DOI: 10.1002/mabi.202000046

The reduction of free radicals by bioactive membranes used for hemodialysis treatment is an important topic due to the constant rise of oxidative stress‐associated cardiovascular mortality by hemodialysis patients. Therefore, it is urgent to find an effective solution that helps to solve this problem. Polysulfone membranes enriched with α‐lipoic acid, α‐tocopherol, and with both components are fabricated by spin coating. The antioxidant properties of these membranes are evaluated in vitro by determining the lipid‐peroxidation level and the total antioxidant status of the blood plasma. The biocompatibility is assessed by quantifying the protein adsorption, platelet adhesion, complement activation, and hemolytic effect. All types of membranes show in vitro antioxidant activity and a trend to reduce oxidative stress in vivo; the best results show membranes prepared with a combination of both compounds and prove to be nonhemolytic and hemocompatible. Moreover, the membrane specific separation ability for the main waste products is not affected by antioxidants incorporation. Treatment of kidney disease patients with conventional hemodialysis membranes causes significantly elevated oxidative stress, associated with severe complications. In order to reduce reactive oxygen species formed during hemodialysis, polysulfone flat sheet membranes modified with antioxidants alpha‐lipoic acid, alpha‐tocopherol, and a combination of both are developed. All three types of membranes show antioxidant activity and the ability to reduce oxidative stress, while maintaining biocompatibility.