Concentration‐dependent effects of latex F1‐protein fraction incorporated into deproteinized bovine bone and biphasic calcium phosphate on the repair of critical‐size bone defects

F1‐protein fraction (F1) is a natural bioactive compound extracted from the rubber tree, Hevea brasiliensis, and has been recently studied for its therapeutic potential in wound healing. In this study, we investigated the concentration‐dependent effects of F1 (0.01%, 0.025%, 0.05%, and 0.1%) incorpo...

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Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2020-11, Vol.108 (8), p.3270-3285
Main Authors: Paini, Suelen, Bighetti, Ana Carolina Cestari, Cestari, Tania Mary, Arantes, Ricardo Vinicius Nunes, Santos, Paula Sanches, Mena‐Laura, Ever Elias, Garlet, Gustavo Pompermaier, Taga, Rumio, Assis, Gerson Francisco
Format: Article
Language:English
Subjects:
Bioactive compounds
Biocompatibility
Biological activity
Biomedical materials
Bone growth
Bone histomorphometry
bone repair
Calcium phosphates
Defects
deproteinized bovine bone
Latex
Materials research
Materials science
Microtomography
natural latex protein fraction
Osteogenesis
porous biphasic calcium phosphate
protein adsorption
Proteins
rat calvarial critical‐size bone defects
Repair
Rubber trees
Tissue engineering
Two dimensional analysis
Wound healing
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Summary:F1‐protein fraction (F1) is a natural bioactive compound extracted from the rubber tree, Hevea brasiliensis, and has been recently studied for its therapeutic potential in wound healing. In this study, we investigated the concentration‐dependent effects of F1 (0.01%, 0.025%, 0.05%, and 0.1%) incorporated into deproteinized bovine bone (DBB) and porous biphasic calcium phosphate (pBCP), on the repair of rat calvarial critical‐size bone defects (CSBD). The defects were analyzed by 3D‐microtomography and 2D‐histomorphometry at 12 weeks postsurgery. The binding efficiency of F1 to pBCP (96.3 ± 1.4%) was higher than that to DBB (67.7 ± 3.3%). In vivo analysis showed a higher bone volume (BV) gain in all defects treated with DBB (except in 0.1% of F1) and pBCP (except in 0.05% and 0.1% of F1) compared to the CSBD without treatment/control group (9.96 ± 2.8 mm3). DBB plus 0.025% F1 promoted the highest BV gain (29.7 ± 2.2 mm3, p  .05). Additionally, a small BV occurred in defects treated with pBCP plus 0.1% F1 (10.4 ± 1.4 mm3, p
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.34664