Steroid‐depleted endometriosis serum improves oocyte maturation in IVM systems

In vitro maturation (IVM) is a novel approach to overcome the adverse effects of human in vitro fertilization (IVF). The aim of the present study is to evaluate the effect of total and steroid‐depleted serum obtained from patients with endometriosis on IVM outcome as supplementation for this system....

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Bibliographic Details
Published in:Journal of cellular physiology 2021-01, Vol.236 (1), p.205-214
Main Authors: Asghari, Samira, Nouri, Mohammad, Darabi, Masoud, Valizadeh, Amir
Format: Article
Language:English
Subjects:
Apoptosis
COX‐2
Depletion
Desaturase
Endometriosis
Fatty acids
Flow cytometry
Gametocytes
Gene expression
In vitro fertilization
in vitro maturation
Liquid chromatography
Maturation
Oocytes
Palmitic acid
Prostaglandin endoperoxide synthase
SCD1
Stearoyl-CoA desaturase
Steroids
steroid‐depleted serum
Supplements
Synthesis
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Summary:In vitro maturation (IVM) is a novel approach to overcome the adverse effects of human in vitro fertilization (IVF). The aim of the present study is to evaluate the effect of total and steroid‐depleted serum obtained from patients with endometriosis on IVM outcome as supplementation for this system. To this purpose, patients with endometriosis were selected according to in/excluding criteria. Germinal vesicles (GVs) and cumulus cells were treated with 10% of each serum. The expression levels of stearoyl CoA desaturase 1 (SCD 1) and cyclooxygenase‐2 (COX‐2) genes were evaluated by RT‐qPCR. Gas‐liquid chromatography and flow cytometry were performed to analyze fatty acids composition and apoptosis. The mRNA expression levels of SCD1 (2.47 fold) and COX‐2 (6.4 fold), and also the synthesis of oleate, linoleate, and arachidonate were increased (1.19, 1.06, and 2.37 folds, respectively) in cumulus cells treated with steroid‐depleted serum (p < .05). The synthesis of palmitate, palmitoleate, and stearate (0.995, 0.67, and 0.7 folds, respectively) and also the rate of apoptosis were significantly decreased in these cells (p < .05). Moreover, GVs cultured in steroid‐depleted group showed a significantly higher rate of maturation (p < .001). Overall, our findings imply a new insight into the expansion of IVM system in oocytes development.
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.29834