Dysregulation in the expression of (lncRNA-TSIX, TP53INP2 mRNA, miRNA-1283) in spinal cord injury

The objective of this study is to examine the alterations in the levels of expression of serum lncRNA-TSIX, TP53INP2 mRNA, miRNA-1283 in spinal cord injured (SCI) patients versus healthy control. The expression of the selected RNAs in the sera was determined in 23 patients suffering from acute spina...

Full description

Saved in:
Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2020-09, Vol.112 (5), p.3315-3321
Main Authors: Salah, Sara Mohammed Mohammed, Matboli, Marwa, Nasser, Hanaa El-Tayeb, Abdelnaiem, Ibrahim Abdelmohsen, Shafei, Ayman El-sayed, EL-Asmer, Mohamed Farid
Format: Article
Language:English
Subjects:
lncRNA
miRNA
mRNA
Serum
Spinal cord injury
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The objective of this study is to examine the alterations in the levels of expression of serum lncRNA-TSIX, TP53INP2 mRNA, miRNA-1283 in spinal cord injured (SCI) patients versus healthy control. The expression of the selected RNAs in the sera was determined in 23 patients suffering from acute spinal cord injury, 41 individuals with chronic spinal cord injury, and 36 healthy control using real-time reverse-transcription polymerase chain reaction method. The results showed that lncRNA-TSIX and the TP53INP2 mRNA expression levels in SCI patients was overexpressed in comparison to the control group alongside with a significant downregulation of miR-1283. Statistically,there was a highly significant positive correlation between lnc-RNA-TRIX and TP53INP2 mRNA with inverse correlation between miRNA-1283 and lnc-RNA-TRIX based on fold changes. Up-regulation of lncRNA-TSIX, TP53INP2 mRNA with downregulation of miRNA-1283 might be closely associated with progression of SCI. •Serum RNAs may provide an idea about severity and prognosis of SCI•We selected RNA network linked to SCI via in silico approach.•We have measure the differential expression of the selected RNA in SCI patient.•Increase in lncRNA-TSIX, TP53INP2 mRNA with decrease in miRNA-1283 in SCI.
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2020.06.018