Single tube multiplex PCR assay for the identification of banned meat species

Food adulteration has a direct impact on public health, religious faith, fair-trades, and wildlife. In the present study, a reliable and sensitive assay has been developed for verifying meat adulteration in food chain. The multiplex PCR system was optimised for identification of chicken, cow/buffalo...

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Published in:Food additives & contaminants Part B, Surveillance communications Surveillance communications, 2020-10, Vol.13 (4), p.284-291
Main Authors: Iqbal, Memoona, Saleem, Muhammad Sulyman, Imran, Muhammad, Khan, Waseem Ahmad, Ashraf, Kamran, Yasir Zahoor, M., Rashid, Imran, Rehman, Habib-Ur, Nadeem, Asif, Ali, Saadat, Naz, Sarwat, Shehzad, Wasim
Format: Article
Language:English
Subjects:
12S r RNA
admixture
adulteration
Diagnostic systems
Electrophoresis
Food chains
Gene sequencing
Inspection
Livestock
Meat
Meat products
meat species
Multiplex PCR
Multiplexing
Pakistan
Pork
Public health
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Summary:Food adulteration has a direct impact on public health, religious faith, fair-trades, and wildlife. In the present study, a reliable and sensitive assay has been developed for verifying meat adulteration in food chain. The multiplex PCR system was optimised for identification of chicken, cow/buffalo, sheep/goat, horse/donkey, pork, and dog DNAs in a single reaction mixture simultaneously. The primers were designed using 12 S rRNA gene sequences with fragment size in the range of 113 bp to 800 bp, which can be easily visualised on agarose gel electrophoresis making the technique economical. After validation of accuracy, specificity, and sensitivity, commercially available meat products (n = 190) were screened, comprising both raw and cooked meat samples. The results demonstrated a high rate of adulteration (54.5%) in meat products. The technique developed here can be easily used for screening of different meat products for export and import purposes as well as for food inspection and livestock diagnostic laboratories.
ISSN:1939-3210
1939-3229
DOI:10.1080/19393210.2020.1778098