Faecal Biomarkers in Inflammatory Bowel Diseases: Calprotectin Versus Lipocalin-2—a Comparative Study

Abstract Background and Aims Faecal biomarkers, particularly calprotectin [FCAL], have become important diagnostic and monitoring tools in inflammatory bowel diseases [IBD]. As FCAL is mainly produced by neutrophils, we hypothesised that faecal lipocalin-2 [FLCN2], also expressed by intestinal epith...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Crohn's and colitis 2021-01, Vol.15 (1), p.43-54
Main Authors: Zollner, Andreas, Schmiderer, Andreas, Reider, Simon J, Oberhuber, Georg, Pfister, Alexandra, Texler, Bernhard, Watschinger, Christina, Koch, Robert, Effenberger, Maria, Raine, Tim, Tilg, Herbert, Moschen, Alexander R
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c335t-c723cf0436adf5a5771944af51e2c03aefdca6efa645503689089dc68b164cc03
cites cdi_FETCH-LOGICAL-c335t-c723cf0436adf5a5771944af51e2c03aefdca6efa645503689089dc68b164cc03
container_end_page 54
container_issue 1
container_start_page 43
container_title Journal of Crohn's and colitis
container_volume 15
creator Zollner, Andreas
Schmiderer, Andreas
Reider, Simon J
Oberhuber, Georg
Pfister, Alexandra
Texler, Bernhard
Watschinger, Christina
Koch, Robert
Effenberger, Maria
Raine, Tim
Tilg, Herbert
Moschen, Alexander R
description Abstract Background and Aims Faecal biomarkers, particularly calprotectin [FCAL], have become important diagnostic and monitoring tools in inflammatory bowel diseases [IBD]. As FCAL is mainly produced by neutrophils, we hypothesised that faecal lipocalin-2 [FLCN2], also expressed by intestinal epithelial cells [IEC], could be beneficial in specific clinical situations. Methods We compared clinical and endoscopic activity-related correlations between FCAL and FLCN2, assayed from the same sample, in a cohort of 132 patients (72 Crohn’s disease [CD]) and 40 controls. A detailed analysis of cellular origins was done by confocal microscopy and flow cytometry. To evaluate the potential to detect low-grade inflammation, we studied faecal and tissue concentrations in a cohort with clinical, endoscopic, and histological remission. Results There was an excellent correlation between FCAL and FLCN2 [rS = 0.87, p
doi_str_mv 10.1093/ecco-jcc/jjaa124
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2415291473</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/ecco-jcc/jjaa124</oup_id><sourcerecordid>2415291473</sourcerecordid><originalsourceid>FETCH-LOGICAL-c335t-c723cf0436adf5a5771944af51e2c03aefdca6efa645503689089dc68b164cc03</originalsourceid><addsrcrecordid>eNptkLtOwzAUhi0Eotx2JuQRCYXa8SUxGy23SpUYuKzRwTlBKUkd4gTUjYfgCXkSDC2Igcln-P5P1kfIPmfHnBkxRGtdNLN2OJsB8FiukS2eJjqSMjHr37eIjJF6QLa9nzGmjErSTTIQsVJa8GSLPF4AWqjoqHQ1tE_YelrO6WReVFDX0Ll2QUfuFSt6VnoEj_6EjqFqWteh7QJ5Hxa9p9OycUFTzqP44-0d6NjVDbTQlS9Ib7o-X-ySjQIqj3urd4fcXZzfjq-i6fXlZHw6jawQqotsEgtbMCk05IUClSTcSAmF4hhbJgCL3ILGArRUigmdGpaa3Or0gWtpA7FDDpfe8MXnHn2X1aW3WFUwR9f7LJZcxYbLRASULVHbOu9bLLKmLUOERcZZ9pU3-8qbhbzZKm-YHKzs_UON-e_gp2cAjpaA65t_ddFf3ScfIImB</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2415291473</pqid></control><display><type>article</type><title>Faecal Biomarkers in Inflammatory Bowel Diseases: Calprotectin Versus Lipocalin-2—a Comparative Study</title><source>Oxford Journals Online</source><creator>Zollner, Andreas ; Schmiderer, Andreas ; Reider, Simon J ; Oberhuber, Georg ; Pfister, Alexandra ; Texler, Bernhard ; Watschinger, Christina ; Koch, Robert ; Effenberger, Maria ; Raine, Tim ; Tilg, Herbert ; Moschen, Alexander R</creator><creatorcontrib>Zollner, Andreas ; Schmiderer, Andreas ; Reider, Simon J ; Oberhuber, Georg ; Pfister, Alexandra ; Texler, Bernhard ; Watschinger, Christina ; Koch, Robert ; Effenberger, Maria ; Raine, Tim ; Tilg, Herbert ; Moschen, Alexander R</creatorcontrib><description>Abstract Background and Aims Faecal biomarkers, particularly calprotectin [FCAL], have become important diagnostic and monitoring tools in inflammatory bowel diseases [IBD]. As FCAL is mainly produced by neutrophils, we hypothesised that faecal lipocalin-2 [FLCN2], also expressed by intestinal epithelial cells [IEC], could be beneficial in specific clinical situations. Methods We compared clinical and endoscopic activity-related correlations between FCAL and FLCN2, assayed from the same sample, in a cohort of 132 patients (72 Crohn’s disease [CD]) and 40 controls. A detailed analysis of cellular origins was done by confocal microscopy and flow cytometry. To evaluate the potential to detect low-grade inflammation, we studied faecal and tissue concentrations in a cohort with clinical, endoscopic, and histological remission. Results There was an excellent correlation between FCAL and FLCN2 [rS = 0.87, p &lt;0.001] and comparable sensitivity and specificity to predict clinical and endoscopic disease activity, with optimal thresholds for endoscopic activity of 73.4 and 1.98 µg/g in ulcerative colitis [UC] and 78.4 and 0.56 µg/g in Crohn’s disease for FCAL and FLCN2, respectively. Strong co-expression of both proteins was observed in granulocytes and macrophages. IECs expressed LCN2 but not CAL. In our IBD cohort in deep remission neither FCAL nor FLCN2 was different from controls; yet mucosal LCN2 but not CAL expressions remained elevated in the rectum of UC and the ileum of CD patients. Conclusions This study corroborates the diagnostic equivalence of FLCN2 and FCAL in IBD. In remission, persistent mucosal overexpression renders LCN2 an attractive candidate for molecular inflammation warranting further investigation.</description><identifier>ISSN: 1873-9946</identifier><identifier>EISSN: 1876-4479</identifier><identifier>DOI: 10.1093/ecco-jcc/jjaa124</identifier><identifier>PMID: 32556317</identifier><language>eng</language><publisher>UK: Oxford University Press</publisher><ispartof>Journal of Crohn's and colitis, 2021-01, Vol.15 (1), p.43-54</ispartof><rights>The Author(s) 2020. Published by Oxford University Press on behalf of European Crohn’s and Colitis Organisation. All rights reserved. For permissions, please email: journals.permissions@oup.com 2020</rights><rights>The Author(s) 2020. Published by Oxford University Press on behalf of European Crohn’s and Colitis Organisation. All rights reserved. For permissions, please email: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c335t-c723cf0436adf5a5771944af51e2c03aefdca6efa645503689089dc68b164cc03</citedby><cites>FETCH-LOGICAL-c335t-c723cf0436adf5a5771944af51e2c03aefdca6efa645503689089dc68b164cc03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32556317$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zollner, Andreas</creatorcontrib><creatorcontrib>Schmiderer, Andreas</creatorcontrib><creatorcontrib>Reider, Simon J</creatorcontrib><creatorcontrib>Oberhuber, Georg</creatorcontrib><creatorcontrib>Pfister, Alexandra</creatorcontrib><creatorcontrib>Texler, Bernhard</creatorcontrib><creatorcontrib>Watschinger, Christina</creatorcontrib><creatorcontrib>Koch, Robert</creatorcontrib><creatorcontrib>Effenberger, Maria</creatorcontrib><creatorcontrib>Raine, Tim</creatorcontrib><creatorcontrib>Tilg, Herbert</creatorcontrib><creatorcontrib>Moschen, Alexander R</creatorcontrib><title>Faecal Biomarkers in Inflammatory Bowel Diseases: Calprotectin Versus Lipocalin-2—a Comparative Study</title><title>Journal of Crohn's and colitis</title><addtitle>J Crohns Colitis</addtitle><description>Abstract Background and Aims Faecal biomarkers, particularly calprotectin [FCAL], have become important diagnostic and monitoring tools in inflammatory bowel diseases [IBD]. As FCAL is mainly produced by neutrophils, we hypothesised that faecal lipocalin-2 [FLCN2], also expressed by intestinal epithelial cells [IEC], could be beneficial in specific clinical situations. Methods We compared clinical and endoscopic activity-related correlations between FCAL and FLCN2, assayed from the same sample, in a cohort of 132 patients (72 Crohn’s disease [CD]) and 40 controls. A detailed analysis of cellular origins was done by confocal microscopy and flow cytometry. To evaluate the potential to detect low-grade inflammation, we studied faecal and tissue concentrations in a cohort with clinical, endoscopic, and histological remission. Results There was an excellent correlation between FCAL and FLCN2 [rS = 0.87, p &lt;0.001] and comparable sensitivity and specificity to predict clinical and endoscopic disease activity, with optimal thresholds for endoscopic activity of 73.4 and 1.98 µg/g in ulcerative colitis [UC] and 78.4 and 0.56 µg/g in Crohn’s disease for FCAL and FLCN2, respectively. Strong co-expression of both proteins was observed in granulocytes and macrophages. IECs expressed LCN2 but not CAL. In our IBD cohort in deep remission neither FCAL nor FLCN2 was different from controls; yet mucosal LCN2 but not CAL expressions remained elevated in the rectum of UC and the ileum of CD patients. Conclusions This study corroborates the diagnostic equivalence of FLCN2 and FCAL in IBD. In remission, persistent mucosal overexpression renders LCN2 an attractive candidate for molecular inflammation warranting further investigation.</description><issn>1873-9946</issn><issn>1876-4479</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNptkLtOwzAUhi0Eotx2JuQRCYXa8SUxGy23SpUYuKzRwTlBKUkd4gTUjYfgCXkSDC2Igcln-P5P1kfIPmfHnBkxRGtdNLN2OJsB8FiukS2eJjqSMjHr37eIjJF6QLa9nzGmjErSTTIQsVJa8GSLPF4AWqjoqHQ1tE_YelrO6WReVFDX0Ll2QUfuFSt6VnoEj_6EjqFqWteh7QJ5Hxa9p9OycUFTzqP44-0d6NjVDbTQlS9Ib7o-X-ySjQIqj3urd4fcXZzfjq-i6fXlZHw6jawQqotsEgtbMCk05IUClSTcSAmF4hhbJgCL3ILGArRUigmdGpaa3Or0gWtpA7FDDpfe8MXnHn2X1aW3WFUwR9f7LJZcxYbLRASULVHbOu9bLLKmLUOERcZZ9pU3-8qbhbzZKm-YHKzs_UON-e_gp2cAjpaA65t_ddFf3ScfIImB</recordid><startdate>20210113</startdate><enddate>20210113</enddate><creator>Zollner, Andreas</creator><creator>Schmiderer, Andreas</creator><creator>Reider, Simon J</creator><creator>Oberhuber, Georg</creator><creator>Pfister, Alexandra</creator><creator>Texler, Bernhard</creator><creator>Watschinger, Christina</creator><creator>Koch, Robert</creator><creator>Effenberger, Maria</creator><creator>Raine, Tim</creator><creator>Tilg, Herbert</creator><creator>Moschen, Alexander R</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210113</creationdate><title>Faecal Biomarkers in Inflammatory Bowel Diseases: Calprotectin Versus Lipocalin-2—a Comparative Study</title><author>Zollner, Andreas ; Schmiderer, Andreas ; Reider, Simon J ; Oberhuber, Georg ; Pfister, Alexandra ; Texler, Bernhard ; Watschinger, Christina ; Koch, Robert ; Effenberger, Maria ; Raine, Tim ; Tilg, Herbert ; Moschen, Alexander R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-c723cf0436adf5a5771944af51e2c03aefdca6efa645503689089dc68b164cc03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zollner, Andreas</creatorcontrib><creatorcontrib>Schmiderer, Andreas</creatorcontrib><creatorcontrib>Reider, Simon J</creatorcontrib><creatorcontrib>Oberhuber, Georg</creatorcontrib><creatorcontrib>Pfister, Alexandra</creatorcontrib><creatorcontrib>Texler, Bernhard</creatorcontrib><creatorcontrib>Watschinger, Christina</creatorcontrib><creatorcontrib>Koch, Robert</creatorcontrib><creatorcontrib>Effenberger, Maria</creatorcontrib><creatorcontrib>Raine, Tim</creatorcontrib><creatorcontrib>Tilg, Herbert</creatorcontrib><creatorcontrib>Moschen, Alexander R</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Crohn's and colitis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zollner, Andreas</au><au>Schmiderer, Andreas</au><au>Reider, Simon J</au><au>Oberhuber, Georg</au><au>Pfister, Alexandra</au><au>Texler, Bernhard</au><au>Watschinger, Christina</au><au>Koch, Robert</au><au>Effenberger, Maria</au><au>Raine, Tim</au><au>Tilg, Herbert</au><au>Moschen, Alexander R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Faecal Biomarkers in Inflammatory Bowel Diseases: Calprotectin Versus Lipocalin-2—a Comparative Study</atitle><jtitle>Journal of Crohn's and colitis</jtitle><addtitle>J Crohns Colitis</addtitle><date>2021-01-13</date><risdate>2021</risdate><volume>15</volume><issue>1</issue><spage>43</spage><epage>54</epage><pages>43-54</pages><issn>1873-9946</issn><eissn>1876-4479</eissn><abstract>Abstract Background and Aims Faecal biomarkers, particularly calprotectin [FCAL], have become important diagnostic and monitoring tools in inflammatory bowel diseases [IBD]. As FCAL is mainly produced by neutrophils, we hypothesised that faecal lipocalin-2 [FLCN2], also expressed by intestinal epithelial cells [IEC], could be beneficial in specific clinical situations. Methods We compared clinical and endoscopic activity-related correlations between FCAL and FLCN2, assayed from the same sample, in a cohort of 132 patients (72 Crohn’s disease [CD]) and 40 controls. A detailed analysis of cellular origins was done by confocal microscopy and flow cytometry. To evaluate the potential to detect low-grade inflammation, we studied faecal and tissue concentrations in a cohort with clinical, endoscopic, and histological remission. Results There was an excellent correlation between FCAL and FLCN2 [rS = 0.87, p &lt;0.001] and comparable sensitivity and specificity to predict clinical and endoscopic disease activity, with optimal thresholds for endoscopic activity of 73.4 and 1.98 µg/g in ulcerative colitis [UC] and 78.4 and 0.56 µg/g in Crohn’s disease for FCAL and FLCN2, respectively. Strong co-expression of both proteins was observed in granulocytes and macrophages. IECs expressed LCN2 but not CAL. In our IBD cohort in deep remission neither FCAL nor FLCN2 was different from controls; yet mucosal LCN2 but not CAL expressions remained elevated in the rectum of UC and the ileum of CD patients. Conclusions This study corroborates the diagnostic equivalence of FLCN2 and FCAL in IBD. In remission, persistent mucosal overexpression renders LCN2 an attractive candidate for molecular inflammation warranting further investigation.</abstract><cop>UK</cop><pub>Oxford University Press</pub><pmid>32556317</pmid><doi>10.1093/ecco-jcc/jjaa124</doi><tpages>12</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1873-9946
ispartof Journal of Crohn's and colitis, 2021-01, Vol.15 (1), p.43-54
issn 1873-9946
1876-4479
language eng
recordid cdi_proquest_miscellaneous_2415291473
source Oxford Journals Online
title Faecal Biomarkers in Inflammatory Bowel Diseases: Calprotectin Versus Lipocalin-2—a Comparative Study
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T05%3A14%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Faecal%20Biomarkers%20in%20Inflammatory%20Bowel%20Diseases:%20Calprotectin%20Versus%20Lipocalin-2%E2%80%94a%20Comparative%20Study&rft.jtitle=Journal%20of%20Crohn's%20and%20colitis&rft.au=Zollner,%20Andreas&rft.date=2021-01-13&rft.volume=15&rft.issue=1&rft.spage=43&rft.epage=54&rft.pages=43-54&rft.issn=1873-9946&rft.eissn=1876-4479&rft_id=info:doi/10.1093/ecco-jcc/jjaa124&rft_dat=%3Cproquest_cross%3E2415291473%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c335t-c723cf0436adf5a5771944af51e2c03aefdca6efa645503689089dc68b164cc03%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2415291473&rft_id=info:pmid/32556317&rft_oup_id=10.1093/ecco-jcc/jjaa124&rfr_iscdi=true