Analysis of the expression profile of long non‐coding RNAs MALAT1 and THRIL in children with immune thrombocytopenia

Background/Aims Pediatric immune thrombocytopenia (ITP) is an autoimmune disease; whose etiology is not exactly understood and seems to be highly multifactorial. Long non‐coding RNAs (lncRNAs) are key regulators of different actions, which contribute to the development of many autoimmune diseases. T...

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Bibliographic Details
Published in:IUBMB life 2020-09, Vol.72 (9), p.1941-1950
Main Authors: Ayoub, Shymaa E., Hefzy, Enas M., Abd El‐Hmid, Rehab G., Ahmed, Naglaa A., Khalefa, Abeer A., Ali, Doaa Y., Ali, Marwa A.
Format: Article
Language:English
Subjects:
Adenocarcinoma
Autoimmune diseases
Biomarkers
Children
Etiology
Idiopathic thrombocytopenic purpura
immune thrombocytopenia
ITP
long non‐coding RNA (lncRNA)
MALAT1
Metastases
Non-coding RNA
Patients
Pediatrics
THRIL
Thrombocytopenia
Transcription
Tumor necrosis factor
Tumor necrosis factor-TNF
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Summary:Background/Aims Pediatric immune thrombocytopenia (ITP) is an autoimmune disease; whose etiology is not exactly understood and seems to be highly multifactorial. Long non‐coding RNAs (lncRNAs) are key regulators of different actions, which contribute to the development of many autoimmune diseases. To gain a further understanding, we estimated the relative expression of lncRNAs Metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) and tumor necrosis factor‐α (TNF‐α) and heterogeneous nuclear ribonucleoprotein L (hnRNPL) immune‐regulatory lncRNA (THRIL) in pediatric ITP. Methods In this case‐control study, analysis of the expression profiles of these lncRNAs in blood samples from children with ITP and healthy controls (HCs) using quantitative real‐time PCR was done. The association of MALAT1 and THRIL with ITP clinical features and their potential usage as non‐invasive circulating biomarkers for ITP diagnosis was also evaluated. The receiver operating characteristic curve was constructed, and an area under the curve was analyzed. Results Both lncRNAs MALAT1 and THRIL were significantly upregulated in ITP patients in comparison to HCs ( p < .0001 and = .001 respectively). In addition, there was a positive significant correlation between the expression level of both biomarkers among patients (r = 0.745, p
ISSN:1521-6543
1521-6551
DOI:10.1002/iub.2310