Modulation of photorespiration and nitrogen recycling in Fe-deficient cucumber leaves

Low Fe availability affects plant production mainly by impairing the photosynthetic pathway, since Fe plays an essential role in chlorophyll synthesis as well as in the photosynthetic electron transport chain. Under these conditions, plant cells require the activation of protective mechanisms to pre...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology and biochemistry 2020-09, Vol.154, p.142-150
Main Authors: Casiraghi, Fabio M., Landi, Marco, Donnini, Silvia, Borlotti, Andrea, Zocchi, Graziano, Guidi, Lucia, Vigani, Gianpiero
Format: Article
Language:English
Subjects:
Cucumis sativus - metabolism
Fe deficiency
Glutamate-Ammonia Ligase - metabolism
Iron - deficiency
Iron metabolism
Nitrate Reductase
Nitrogen - metabolism
Peroxisome
Photorespiration
Photosynthesis
Plant Leaves - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Low Fe availability affects plant production mainly by impairing the photosynthetic pathway, since Fe plays an essential role in chlorophyll synthesis as well as in the photosynthetic electron transport chain. Under these conditions, plant cells require the activation of protective mechanisms to prevent photo-inhibition. Among these mechanisms, photorespiration (PR) has been relatively little investigated in Fe-deficient plants. The aim of this work was to investigate the effect of Fe deficiency on photorespiration by performing in vivo analysis in leaves as well as biochemical characterization of some PR-related enzyme activities in a peroxisome-purified fraction from cucumber leaves. Modelling of light response curves at both 21 and 2% pO2 revealed a slowing down of PR under Fe deficiency. The activity of some PR-involving enzymes as well as the contents of glycine and serine were affected under Fe deficiency. Furthermore, nitrate reductase, the glutamine synthetase-glutamate synthase (GS-GOGAT) cycle and hydroxypyruvate dehydrogenase isoform activities were differentially altered under Fe deficiency. The dataset indicates that, in Fe-deficient cucumber leaves, the modulation of PR involves the induction of some PR-related pathways, such as the photorespiratory N recycling and cytosolic photorespiratory bypass processes. •The effect of Fe deficiency on photorespiration has been little investigated.•Photorespiration is affected by low Fe availability in cucumber leaves.•Biochemical investigation revealed changes in peroxisome-enzymes.•PR modulation involves the induction of N-recycling in Fe-deficient leaf.
ISSN:0981-9428
1873-2690
DOI:10.1016/j.plaphy.2020.05.032