Coexpression of VGLUT1 and VGLUT2 in precerebellar neurons in the lateral reticular nucleus of the rat

• Published in: Brain research bulletin 2020-09, Vol.162, p.94-106
• Main Authors: Li, Zhi-Hong, Zhang, Chun-Kui, Qiao, Yu, Ge, Shun-Nan, Zhang, Ting, Li, Jin-Lian
• Format: Article
• Language: English
• Subjects: Cerebellum; Lateral reticular nucleus; Mossy fiber; Tract-tracing; Vesicular glutamate transporter
• ISSN: 0361-9230; 1873-2747
• DOI: 10.1016/j.brainresbull.2020.06.008

•Coexpression of VGLUT1 and VGLUT2 mRNA in lateral reticular nucleus.•Cerebellar-projecting neurons in lateral reticular nucleus are VGLUT1 or VGLUT2 positive.•BDA labeled Mossy-fiber terminal-like structures are both VGLUT1 and VGLUT2 positive.•Disturbed forelimb movement after knocking down VGLUT1 or VGLUT2 in lateral reticular nucleus. Vesicular glutamate transporter (VGLUT) 1 and VGLUT2 have been reported to distribute complementally in most brain regions and have been assumed to define distinct functional elements. Previous studies have shown the expression of VGLUT1 mRNA and VGLUT2 mRNA in the lateral reticular nucleus (LRN), a key precerebellar nucleus sending mossy fibers to the cerebellum. In the present study, we firstly examined the coexpression of VGLUT1 and VGLUT2 mRNA in the LRN of the rat by dual-fluorescence in situ hybridization. About 81.89 % of glutamatergic LRN neurons coexpressed VGLUT1 and VGLUT2 mRNA, and the others expressed either VGLUT1 or VGLUT2 mRNA. We then injected the retrograde tracer Fluogold (FG) into the vermal cortex of cerebellum, and observed that 95.01 % and 86.80 % of FG-labeled LRN neurons expressed VGLUT1 or VGLUT2 mRNA respectively. We further injected the anterograde tracer biotinylated dextran amine (BDA) into the LRN, and found about 82.6 % of BDA labeled axon terminals in the granular layer of cerebellar cortex showed both VGLUT1- and VGLUT2-immunoreactivities. Afterwards, we observed under electron microscopy that anterogradely labeled axon terminals showing immunoreactivity for VGLUT1 or VGLUT2 made asymmetric synapses with dendritic profiles of cerebellar neurons. Finally, we selectively down-regulated the expression of VGLUT1 mRNA or VGLUT2 mRNA by using viral vector mediated siRNA transfection and detected that the fine movements of the forelimb of rats were disturbed. These results indicated that LRN neurons coexpressing VGLUT1 and VGLUT2 project to the cerebellar cortex and these neurons might be critical in mediating the forelimb movements.