Cloning of a SEPALLATA4-like gene (IiSEP4) in Isatis indigotica Fortune and characterization of its function in Arabidopsis thaliana

E-class MADS-box genes, SEPALLATA (SEP), participate in various aspects of plant development together with B-, C- and D-class MADS-box genes. IiSEP4, a homologous gene of SEP4, was cloned from Isatis indigotica. IiSEP4 was highly expressed in sepals, and its mRNA was mildly detected in leaves, inflo...

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Published in:Plant physiology and biochemistry 2020-09, Vol.154, p.229-237
Main Authors: Pu, Zuo-Qian, Ma, Ye-Ye, Lu, Meng-Xin, Ma, Yan-Qin, Xu, Zi-Qin
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis thaliana
Cloning, Molecular
Expression analysis
Floral development
Flowers - physiology
Gene Expression Regulation, Plant
IiSEP4
Isatis - genetics
Isatis indigotica Fortune
MADS Domain Proteins - genetics
MADS Domain Proteins - physiology
Phylogeny
Plant Proteins - genetics
Plant Proteins - physiology
Plants, Genetically Modified
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Summary:E-class MADS-box genes, SEPALLATA (SEP), participate in various aspects of plant development together with B-, C- and D-class MADS-box genes. IiSEP4, a homologous gene of SEP4, was cloned from Isatis indigotica. IiSEP4 was highly expressed in sepals, and its mRNA was mildly detected in leaves, inflorescences, flowers, stamens and young silicles. Constitutive expression of IiSEP4 in Arabidopsis thaliana caused early flowering, accompanied by the reduction of flowers and floral organs. Moreover, the sepals in some flowers were transformed into carpelloid structures with stigmatic papillae, and obviously accompanied by ovule formation. Yeast two-hybrid assays demonstrated that IiSEP4 interacts with other woad MADS proteins to determine the identity of floral organs. These findings reveal the important roles of IiSEP4 in floral development of I. indigotica. The results of this study can lay a foundation for further study on biological functions of MADS transcriptional factors in I. indigotica. •cDNA and genomic DNA sequences of IiSEP4 in Isatis indigotica was determined.•Sepals were transformed into carpelloid structures in IiSEP4 transgenic Arabidopsis.•The epidermal cells in IiSEP4 transgenic petals are similar to that of sepals.•Petal number is reduced and stomata are generated in IiSEP4 transgenic petals.
ISSN:0981-9428
1873-2690
DOI:10.1016/j.plaphy.2020.05.031