Effect of pranlukast on neonatal rats with periventricular leukomalacia

To study the effect of pranlukast (Pran) on neonatal rats with periventricular leukomalacia (PVL). The rats, aged 3 days, were randomly divided into a sham-operation group, a PVL group, and a Pran group. A rat model of PVL was prepared by right common carotid artery ligation and postoperative hypoxi...

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Bibliographic Details
Published in:Zhongguo dang dai er ke za zhi 2020-06, Vol.22 (6), p.656
Main Authors: Ye, Xiao-Yan, Wang, Da-Yu, Xu, Yan, Wang, Jun
Format: Article
Language:Chinese
Subjects:
Animals
Animals, Newborn
Chromones
Leukomalacia, Periventricular
Rats
Receptors, G-Protein-Coupled
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Summary:To study the effect of pranlukast (Pran) on neonatal rats with periventricular leukomalacia (PVL). The rats, aged 3 days, were randomly divided into a sham-operation group, a PVL group, and a Pran group. A rat model of PVL was prepared by right common carotid artery ligation and postoperative hypoxia. The rats in the sham-operation group were given isolation of the right common carotid artery without ligation or hypoxic treatment. The rats in the Pran group were given intraperitoneal injection of Pran (0.1 mg/kg) once every 12 hours, for 3 consecutive days, and those in the sham-operation group and the PVL group were given intraperitoneal injection of an equal volume of normal saline. On day 14 after modeling, hematoxylin-eosin (HE) staining was used to observe the pathological changes of brain tissue; immunofluorescent staining was used to measure the expression of myelin basic protein (MBP) in brain tissue (n=8); Western blot was used to measure the expression of cyclic nucleotide phosphodiesterase (CNPase)
ISSN:1008-8830