Electrostatic effects on the electrical tension-induced irreversible pore formation in giant unilamellar vesicles

[Display omitted] Irreversible electroporation (IRE) is a new technique in which a series of short pulses with high frequency electrical energy is applied on the targeted regions of cells or vesicles for their destruction or rupture formation. IRE induces lateral tension in the membranes of vesicles...

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Published in:Chemistry and physics of lipids 2020-09, Vol.231, p.104935-104935, Article 104935
Main Authors: Karal, Mohammad Abu Sayem, Orchi, Urbi Shyamolima, Towhiduzzaman, Md, Ahamed, Md. Kabir, Ahmed, Marzuk, Ahammed, Shareef, Mokta, Nadia Akter, Sharmin, Sabrina, Sarkar, Malay Kumar
Format: Article
Language:English
Subjects:
Electricity
Electrostatic effects
Giant unilamellar vesicles
Irreversible electroporation
Particle Size
Phosphatidylcholines - chemistry
Phosphatidylglycerols - chemistry
Pore formation
Rate constant
Static Electricity
Unilamellar Liposomes - chemical synthesis
Unilamellar Liposomes - chemistry
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Summary:[Display omitted] Irreversible electroporation (IRE) is a new technique in which a series of short pulses with high frequency electrical energy is applied on the targeted regions of cells or vesicles for their destruction or rupture formation. IRE induces lateral tension in the membranes of vesicles. We have investigated the electrostatic interaction effects on the constant electrical tension-induced rate constant of irreversible pore formation in the membranes of giant unilamellar vesicles (GUVs). The electrostatic interaction has been varied by changing the salt concentration in buffer and the surface charge density of membranes. The membranes of GUVs are synthesized by a mixture of negatively charged lipid dioleoylphosphatidylglycerol (DOPG) and neutral lipid dioleoylphosphatidylcholine (DOPC) using the natural swelling method. The rate constant of pore formation increases with the decrease of salt concentration in buffer along with the increase of surface charge density of membranes. The tension dependent probability of pore formation and the rate constant of pore formation are fitted to the theoretical equation, and obtained the line tension of membranes. The decrease in energy barrier of a prepore due to electrostatic interaction is the key factor causing an increase of rate constant of pore formation.
ISSN:0009-3084
1873-2941
DOI:10.1016/j.chemphyslip.2020.104935