Expression of upstream and downstream targets of mTOR pathway in seven cases of secretory carcinoma of salivary gland origin

Purpose To investigate the expression of upstream and downstream targets of mTOR signalling pathway in the secretory carcinoma of salivary gland origin (SCsg). Methods Seven cases of secretory carcinoma diagnosed by a combination of immunohistochemistry and/or molecular testing were retrieved from o...

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Published in:European archives of oto-rhino-laryngology 2021, Vol.278 (1), p.279-283
Main Authors: Custódio, Marcos, Sedassari, Bruno Tavares, Altemani, Albina, Rodrigues, Maria Fernanda Setúbal Destro, Nunes, Fábio Daumas, de Sousa, Suzana Cantanhede Orsini Machado
Format: Article
Language:English
Subjects:
Carcinoma
Head and Neck Surgery
Humans
Immunohistochemistry
Medicine
Medicine & Public Health
Neurosurgery
Otorhinolaryngology
Phosphorylation
Proto-Oncogene Proteins c-akt - metabolism
Salivary Glands
Short Communication
Signal Transduction
TOR Serine-Threonine Kinases - metabolism
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Summary:Purpose To investigate the expression of upstream and downstream targets of mTOR signalling pathway in the secretory carcinoma of salivary gland origin (SCsg). Methods Seven cases of secretory carcinoma diagnosed by a combination of immunohistochemistry and/or molecular testing were retrieved from our pathology files. For comparison purposes, 27 other salivary carcinomas were selected. Immunohistochemical staining was performed against phospho-Akt, PTEN, phospho-mTOR, phospho-4E-BP, eIF4E and phospho-S6 ribosomal protein. Results With the exception of Akt, all the other proteins were present at some level in the SCsg and in other salivary carcinomas. PTEN was diffusely expressed in 57.1% of SCsg, but only in 14.8% of other salivary carcinomas. mTOR is expressed in more than half of the cases both for SCsg and other salivary tumour types. Most cases of SCsg showed negative expression for S6 ribosomal protein (71.4%) and 4E-BP1 (57.1%). For both groups evaluated, eIF4E was the most expressed protein. Conclusion SCsg shows different expression patterns for the mTOR signalling molecules, but only eIF4E was highly expressed. This may suggest alternative signalling pathways other than Akt and mTOR in this group of tumours.
ISSN:0937-4477
1434-4726
DOI:10.1007/s00405-020-06146-w