Anti‐inflammatory and immunomodulatory effects of Biodentine on human periapical lesion cells in culture

Aim To evaluate the effect of Biodentine eluate on cytotoxicity and production of pro‐ and anti‐inflammatory cytokines and osteodestructive/osteoprotective cytokines in cultures of human periapical lesion cells. Methodology Conditioned Biodentine Medium (CBM) was prepared according to ISO 10993‐12,...

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Published in:International endodontic journal 2020-10, Vol.53 (10), p.1398-1412
Main Authors: Eraković, M., Duka, M., Bekić, M., Tomić, S., Ismaili, B., Vučević, D., Čolić, M.
Format: Article
Language:English
Subjects:
Anti-Inflammatory Agents - pharmacology
Apoptosis
Biodentine
Bone healing
Calcium Compounds
Cell culture
Chemokines
Collagen
Collagenase
Cytokines
Cytotoxicity
Dental cement
Deoxyribonuclease
Enzyme-linked immunosorbent assay
Flow cytometry
Humans
Immunomodulation
Inflammation
Interferon
Lesions
Lymphocytes T
Microparticles
Osteolysis
Osteoprotegerin
periapical lesion
Silicates
Tooth extractions
TRANCE protein
Tumor necrosis factor
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Summary:Aim To evaluate the effect of Biodentine eluate on cytotoxicity and production of pro‐ and anti‐inflammatory cytokines and osteodestructive/osteoprotective cytokines in cultures of human periapical lesion cells. Methodology Conditioned Biodentine Medium (CBM) was prepared according to ISO 10993‐12, by incubating Biodentine in RPMI medium (0.2 g mL−1) for 3 days at 37 °C. CBM contained both released microparticles and leachable soluble components. Inflammatory cells were isolated from 22 human periapical lesions after apicectomy or tooth extraction, by collagenase/DNase digestion, and cultured in several dilutions of CBM. The composition of periapical lesion cells was determined by morphological criteria, cytotoxicity was quantified by MTT and flow cytometric apoptosis/necrosis assays, whereas the levels of produced cytokines in cell culture supernatants were measured by flow cytometry and ELISA. Student t‐test and Friedman test with Dunn's post‐test were used for comparison of parametric and nonparametric variables, respectively. Results Undiluted (100%), 75% and 50% CBM were cytotoxic for periapical lesion cells due to induction of both necrosis (100% CBM) and apoptosis (75% and 50% CBM). Noncytotoxic concentrations of CBM (25%) inhibited the production of pro‐inflammatory cytokines: TNF‐α, (P 
ISSN:0143-2885
1365-2591
DOI:10.1111/iej.13351