Characterization and proliferation capacity of potentially pathogenic fungi in marine and freshwater fish commercial feeds

• Published in: Archives of microbiology 2020-11, Vol.202 (9), p.2379-2390
• Main Authors: Bobadilla-Carrillo, Giovanna Ilieva, Magallón-Servín, Paola, López-Vela, Melissa, Palomino-Hermosillo, Yolotzin Apatzingán, Ramírez-Ramírez, José Carmen, Gutiérrez-Leyva, Ranferi, Ibarra-Castro, Leonardo, Bautista-Rosales, Pedro Ulises
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Ecology; Life Sciences; Microbial Ecology; Microbiology; Original Paper
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-020-01954-4

In the aquaculture industry, the selection and quality of feed are highly relevant because their integrity and management have an impact on the health and development of organisms. In general, feeds contamination depends on storage conditions and formulation. Furthermore, it has been recognized that filamentous fungi are among the most important contaminating agent in formulated feeds. Therefore, the purpose of this research was to identify saprophytic fungi capable of proliferating in commercial feeds, as well as determining their prevalence, extracellular enzymes profile, ability to assimilate carbon sources, and finally their ability to produce aflatoxins. In order to do that, twenty-two fungi were isolated from commercial fish feeds. After, the species Aspergillus chevalieri , A. cristatus , A. sydowii , A. versicolor , A. flavus , A. creber, and Lichtheimia ramosa were identified. These fungi were able to produce extracellular enzymes, such as phosphatases, esterases, proteases, β-glucosidase, and N -acetyl-β-glucosaminidase. The isolated fungi showed no selective behavior in the assimilation of the different carbon sources, showing a strong metabolic diversity. Prevalence percentages above 85% were recorded. Among all fungi studied, A. flavus M3-C1 had the highest production of aflatoxins when this strain was inoculated directly in the feeds (295 ppb). The aflatoxin production by this strain under the experimental setting is above the permitted levels, and it has been established that high levels of aflatoxins in feeds can cause alterations in fish growth as well as the development of cancerous tumors in the liver, in addition to enhancing mortality.