Effects of Methylene Blue on Ovarian Torsion−Detorsion Injury in a Rat Model

• Published in: Journal of pediatric & adolescent gynecology 2020-10, Vol.33 (5), p.506-510
• Main Authors: Ilgen, Orkun, Hortu, Ismet, Ozceltik, Gokay, Yigitturk, Gurkan, Erbas, Oytun, Karadadas, Nedim
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - pharmacology; Female; Humans; Ischemic injury; Malondialdehyde - analysis; Methylene blue; Methylene Blue - pharmacology; Ovarian detorsion; Ovarian torsion; Ovarian Torsion - prevention & control; Ovary - drug effects; Perfusion-reperfusion injury; Rat model; Rats; Rats, Wistar; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Reperfusion Injury - prevention & control
• ISSN: 1083-3188; 1873-4332
• DOI: 10.1016/j.jpag.2020.06.010

Methylene blue (MB) is an antioxidant that ameliorates ischemia-reperfusion injury in several tissues. We analyzed the effects of MB as an inhibitor of torsion–detorsion injury in rat ovaries. Rats were randomly divided into 5 groups. Group 1 was the sham group, in which only laparotomy was performed. Group 2 was the torsion group, with 3 hours of ischemia. Group 3 was the torsion + MB group, with 3 hours of ischemia after MB administration. Group 4 was the torsion–detorsion group, with 3 hours of ischemia and reperfusion. Finally, group 5 was the torsion–detorsion + MB group, with 3 hours of ischemia and MB administration before detorsion/reperfusion. Ovary injuries were histopathologically scored. Malondialdehyde (MDA) and total protein levels in ovarian tissues were determined, and long pentraxin-3 (PTX3) levels were measured in ovarian tissue using an enzyme-linked immunosorbent assay. In comparing group 4 with group 5 and group 2 with 3, histopathological parameters reflecting injury were significantly increased in groups 4 and 2. Group 3 generated increased MDA levels when compared with group 2 (P