Electron transport in Tradescantia leaves acclimated to high and low light: thermoluminescence, PAM-fluorometry, and EPR studies

• Published in: Photosynthesis research 2020-12, Vol.146 (1-3), p.123-141
• Main Authors: Kalmatskaya, Olesya A., Trubitsin, Boris V., Suslichenko, Igor S., Karavaev, Vladimir A., Tikhonov, Alexander N.
• Format: Article
• Language: English
• Subjects: Analysis; Biochemistry; Biomedical and Life Sciences; Chlorophyll; Chloroplasts; Electron spin resonance; Electron transport; Fluorometry; Life Sciences; Light; Light intensity; Original Article; Photons; Photooxidation; Photosynthesis; Photosynthetic apparatus; Photosystem II; Plant Genetics and Genomics; Plant Physiology; Plant Sciences; Radiation
• ISSN: 0166-8595; 1573-5079
• DOI: 10.1007/s11120-020-00767-2

Using thermoluminescence, PAM-fluorometry, and electron paramagnetic resonance (EPR) for assaying electron transport processes in chloroplasts in situ, we have compared photosynthetic characteristics in Tradescantia fluminensis leaves grown under low light (LL, 50–125 µmol photons m −2 s −1 ) or high light (HL, 875–1000 µmol photons m −2 s −1 ) condition. We found differences in the thermoluminescence (TL) spectra of LL- and HL-acclimated leaves. The LL and HL leaves show different proportions of the Q (~ 0 °C) and B (~ 25–30 °C) bands in their TL spectra; the ratios of the “light sums” of the Q and B bands being S Q / S B ≈ 1/1 (LL) and S Q / S B ≈ 1/3 (HL). This suggests the existence of different redox states of electron carriers on the acceptor side of PSII in LL and HL leaves, which may be affected, in particular, by different capacities of their photo-reducible PQ pools. Enhanced content of PQ in chloroplasts of LL leaves may be the reason for an efficient performance of photosynthesis at low irradiance. Kinetic studies of slow induction of Chl a fluorescence and measurements of P 700 photooxidation by EPR demonstrate that HL leaves have faster (about 2 times) response to switching on actinic light as compared to LL leaves grown at moderate irradiation. HL leaves also show higher non-photochemical quenching (NPQ) of Chl a fluorescence. These properties of HL leaves (faster response to light and generation of enhanced NPQ) reflect the flexibility of their photosynthetic apparatus, providing sustainability and rapid response to fluctuations of environmental light intensity and solar stress resistance. Analysis of time-courses of the EPR signals of P 700 + induced by far-red ( λ max = 707 nm), exciting predominantly PSI, and white light, exciting both PSI and PSII, suggests that there is a contribution of cyclic electron flow around PSI to electron flow through PSI in HL leaves. The data obtained are discussed in terms of photosynthetic apparatus sustainability of HL and LL leaves under variable irradiation conditions.