Flavihumibacter soli sp. nov., Isolated from Soil

• Published in: Current microbiology 2020-10, Vol.77 (10), p.3179-3184
• Main Authors: Seo, Ye Lin, Jung, Jaejoon, Khan, Shehzad Abid, Kim, Kyung Hyun, Jeon, Che Ok
• Format: Article
• Language: English
• Subjects: Bacterial Typing Techniques; Bacteroidetes; Biomedical and Life Sciences; Biotechnology; Catalase; DNA, Bacterial - genetics; Fatty Acids; Gene sequencing; Genomes; Life Sciences; Lipids; Menaquinones; Microbiology; New species; Nucleotide sequence; pH effects; Phosphatidylethanolamine; Phylogenetics; Phylogeny; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sodium chloride; Soil; Soil Microbiology; Soils; Vitamin K 2
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-020-02103-2

A novel Gram-stain-positive, strictly aerobic, non-motile, rod-shaped bacterium, designated strain R14T was isolated from steep grove soil and taxonomically characterized using a polyphasic approach. Cells showed oxidase-negative and catalase-positive activities and grew at 25–37 °C (optimum, 30 °C), pH 5.0–8.0 (optimum, pH 6.5), and in the presence of 0–0.5% (w/v) NaCl (optimum, 0%). Phylogenetic trees based on 16S rRNA gene sequences revealed that strain R14T formed a phylogenetic lineage with Flavihumibacter genus members within the family Chitinophagaceae. Comparison of 16S rRNA gene sequences showed that strain R14T shared highest similarities with Flavihumibacter solisilvae 3-3T (97.5%), Flavihumibacter petaseus NBRC 106054T (96.7%), and Flavihumibacter stibioxidans YS-17T (96.1%). The G+C content of strain R14T calculated from its whole genome sequence was 44.6 mol%. Strain R14T contained menaquinone-7 and iso-C 15:0 , iso-C 17:0 3-OH, C 16:0 , C 15:1 ω6c, and iso-C 15:0 -G as the sole respiratory isoprenoid quinone and major cellular fatty acids, respectively. Major polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids, and four unidentified lipids. Based on phylogenetic, phenotypic, and chemotaxonomic characteristics of strain R14T, it could be concluded that strain R14T represents a novel species of the genus Flavihumibacter , for which the name Flavihumibacter soli sp. nov. is proposed. The type strain is R14T (=KACC 21229T = JCM 33473T).