Population genetics and comparative mitogenomic analyses reveal cryptic diversity of Amphioctopus neglectus (Cephalopoda: Octopodidae)

• Published in: Genomics (San Diego, Calif.) Calif.), 2020-11, Vol.112 (6), p.3893-3902
• Main Authors: Tang, Yan, Zheng, Xiaodong, Liu, Haijuan, Sunxie, Feige
• Format: Article
• Language: English
• Subjects: Amphioctopus neglectus; cox1 and cox3 genes; Cryptic diversity; Genetic diversity; Mitogenome
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2020.06.036

This study presented 96 cox1 and 76 cox3 genes of Amphioctopus neglectus populations. Three distinct lineages were formed from phylogenetic trees and networks constructed using haplotypes. Mitogenomes of A. neglectus-a and A. neglectus-b as the representatives of two lineages separated from population genetics were sequenced to compare with A. neglectus at the genome-level. Amphioctopus neglectus-a showed significant differences with A. neglectus, mainly reflected in gene length, intergenic regions and the secondary structure of tandem repeat motifs. Notably, two sequence deletions in mitogenomes of the two representative species were detected in different positions of major non-coding regions, which were the most distinct differences with A. neglectus. Pairwise genetic distances and the phylogenetic analysis supported the relationship of (A. neglectus-a + (A. neglectus + A. neglectus-b)). This study suggested that A. neglectus-a should be considered as a potential cryptic species of this complex, while A. neglectus-b needed further verification to be defined. •The cryptic biodiversity was detected in Amphioctopus neglectus.•Mitogenome of A. neglectus was compared with the representatives of two lineages separated from the complex.•The significant divergences were found in major non-coding regions in three mitogenomes.•Amphioctopus neglectus-a was considered as a potential cryptic species of this complex.