Auxin is required for the long coleoptile trait in japonica rice under submergence

• Rice coleoptile elongation under submergence guarantees fast seedling establishment in the field. We investigated the role of auxin in influencing the capacity of rice to produce a long coleoptile under water. • In order to explore the complexity of auxin’s role in coleoptile elongation, we used g...

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Bibliographic Details
Published in:The New phytologist 2021-01, Vol.229 (1), p.85-93
Main Authors: Nghi, Khac Nhu, Tagliani, Andrea, Mariotti, Lorenzo, Weits, Daan A., Perata, Pierdomenico, Pucciariello, Chiara
Format: Article
Language:English
Subjects:
auxin
Auxins
Biosynthesis
Coleoptile
Confocal microscopy
Cotyledon
Deoxyribonucleic acid
DNA
Elongation
Fluorescence
Gas chromatography
Gene expression
hypoxia
Indoleacetic Acids
Length
Mass spectrometry
Mass spectroscopy
Microscopy
Oryza - genetics
Oryza sativa
Protein transport
Rapid reports
Rice
Seedlings
Submergence
Tandem Mass Spectrometry
Transport
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Summary:• Rice coleoptile elongation under submergence guarantees fast seedling establishment in the field. We investigated the role of auxin in influencing the capacity of rice to produce a long coleoptile under water. • In order to explore the complexity of auxin’s role in coleoptile elongation, we used gene expression analysis, confocal microscopy of an auxin-responsive fluorescent reporter, gas chromatography coupled to tandem mass spectrometry (GC–MS/MS), and T-DNA insertional mutants of an auxin transport protein. • We show that a higher auxin availability in the coleoptile correlates with the final coleoptile length under submergence. We also identified the auxin influx carrier AUX1 as a component influencing this trait under submergence. The coleoptile tip is involved in the final length of rice varieties harbouring a long coleoptile. • Our experimental results indicate that auxin biosynthesis and transport underlies the differential elongation between short and long coleoptile-harbouring japonica rice varieties.
ISSN:0028-646X
1469-8137
DOI:10.1111/nph.16781