Aquatic hypoxia disturbs oriental river prawn (Macrobrachium nipponense) testicular development: A cross-generational study

• Published in: Environmental pollution (1987) 2020-11, Vol.266, p.115093-115093, Article 115093
• Main Authors: Sun, Shengming, Chen, Yinxiang, Hu, Ran
• Format: Article
• Language: English
• Subjects: Gonadal development; Hypoxia; Macrobrachium nipponense; Testis; Transcriptomics
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2020.115093

Recently, we reported that hypoxia disrupts the endocrine system and causes metabolic abnormalities in prawns. Although transgenerational impairment effects of hypoxia have become a hot topic in vertebrate, it is unknown whether hypoxia could exert cross-generational effects on testicular function crustaceans. The present study aimed to investigate hypoxia’s toxic effects on the testicular function of oriental river prawns (Macrobrachium nipponense) and offspring development. Hypoxia disrupted testicular germ cells quality, caused sex hormone imbalance (testosterone and estradiol), and delayed testicular development. The F1 generation derived from male prawns exposed to hypoxia showed retarded embryonic development, and reduced hatching success and larval development, despite not being exposed to hypoxia. Analysis of the transcriptome the F0 generation (exposed to hypoxia) showed that the impaired testicular functions were associated with changes to mitochondrial oxidative phosphorylation, apoptosis, and steroid biosynthesis. Interestingly, quantitative real-time PCR confirmed that hypoxia could significantly suppress the expression of antioxidant and gonad development-related genes in the testis of the F1 generations, with and without continued hypoxia exposures. In addition, paternal exposure to hypoxia could result in a higher production of reactive oxygen species in offspring testis tissue compared with those without hypoxia exposure. The cross-generational effects of testicular function implied that the sustainability of natural freshwater prawn populations would be threatened by chronic hypoxia. [Display omitted] •Hypoxia damaged testicular germ cells and disturbed the balance of sex hormones.•Hypoxia retarded embryonic development, and reduced hatching and larval development.•Testicular dysfunction correlates with apoptosis and gonad development gene changes.•Hypoxia significantly threatens the sustainability of natural freshwater prawns. Parental transfer of hypoxia induced testicular dysfunction of prawns from F0 to F1 offspring was observed.