Activity of Alcohol Dehydrogenase and Aldehyde Dehydrogenase in Lung Cancer Cells

Background: The aim of this study was to define the alterations in the activity of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in normal and cancerous lung cells. Materials and Methods: Lung tissues were taken from 36 patients during surgical resection of cancer. The act...

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Bibliographic Details
Published in:Anticancer research 2020-07, Vol.40 (7), p.3857-3863
Main Authors: ORYWAL, KAROLINA, JELSKI, WOJCIECH, KOZŁOWSKI, MIROSŁAW DARIUSZ, MROCZKO, BARBARA
Format: Article
Language:English
Subjects:
Alcohol dehydrogenase
Aldehyde dehydrogenase
Aldehydes
Biosynthesis
Carcinogenesis
Carcinogens
Dehydrogenase
Dehydrogenases
Depletion
Glutathione
Isoenzymes
Lung cancer
Oxidative stress
Retinoic acid
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Summary:Background: The aim of this study was to define the alterations in the activity of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in normal and cancerous lung cells. Materials and Methods: Lung tissues were taken from 36 patients during surgical resection of cancer. The activities of tested enzymes were measured by spectrofluorometric method (ADH I, ADH II, total ALDH) and photometric method (ADH III, ADH IV, total ADH). Results: The activities of class II and III ADH were significantly lower in lung cancer cells compared to histologically normal lung tissue. Conclusion: Reduced activity of isoenzyme class II ADH may affect disorders in retinoic acid biosynthesis, leading to its deficit. Lower ADH III activity may result in depletion of glutathione, and in initiation of oxidative stress, leading to cancer progression. These data suggest that alterations in ADH isoenzyme activities can contribute to carcinogenesis in human lungs.
ISSN:0250-7005
1791-7530
DOI:10.21873/anticanres.14375