Activity of Alcohol Dehydrogenase and Aldehyde Dehydrogenase in Lung Cancer Cells

Background: The aim of this study was to define the alterations in the activity of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in normal and cancerous lung cells. Materials and Methods: Lung tissues were taken from 36 patients during surgical resection of cancer. The act...

Full description

Saved in:
Bibliographic Details
Published in:Anticancer research 2020-07, Vol.40 (7), p.3857-3863
Main Authors: ORYWAL, KAROLINA, JELSKI, WOJCIECH, KOZŁOWSKI, MIROSŁAW DARIUSZ, MROCZKO, BARBARA
Format: Article
Language:English
Subjects:
Alcohol dehydrogenase
Aldehyde dehydrogenase
Aldehydes
Biosynthesis
Carcinogenesis
Carcinogens
Dehydrogenase
Dehydrogenases
Depletion
Glutathione
Isoenzymes
Lung cancer
Oxidative stress
Retinoic acid
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c349t-85ee6d82c8a9c4eda9b091da78ca7b6740af4582cde7e7ad464a7498b927d35a3
cites
container_end_page 3863
container_issue 7
container_start_page 3857
container_title Anticancer research
container_volume 40
creator ORYWAL, KAROLINA
JELSKI, WOJCIECH
KOZŁOWSKI, MIROSŁAW DARIUSZ
MROCZKO, BARBARA
description Background: The aim of this study was to define the alterations in the activity of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in normal and cancerous lung cells. Materials and Methods: Lung tissues were taken from 36 patients during surgical resection of cancer. The activities of tested enzymes were measured by spectrofluorometric method (ADH I, ADH II, total ALDH) and photometric method (ADH III, ADH IV, total ADH). Results: The activities of class II and III ADH were significantly lower in lung cancer cells compared to histologically normal lung tissue. Conclusion: Reduced activity of isoenzyme class II ADH may affect disorders in retinoic acid biosynthesis, leading to its deficit. Lower ADH III activity may result in depletion of glutathione, and in initiation of oxidative stress, leading to cancer progression. These data suggest that alterations in ADH isoenzyme activities can contribute to carcinogenesis in human lungs.
doi_str_mv 10.21873/anticanres.14375
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2420152146</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2420152146</sourcerecordid><originalsourceid>FETCH-LOGICAL-c349t-85ee6d82c8a9c4eda9b091da78ca7b6740af4582cde7e7ad464a7498b927d35a3</originalsourceid><addsrcrecordid>eNpdkEtLAzEUhYMoWKs_wN2AGzdTk8lrsiz1CQURdD3cJnfaKdOkJjNC_73TVhC6unDPx-HwEXLL6KRgpeYP4LvGgo-YJkxwLc_IiGnDci05PScjWkiaa0rlJblKaU2pUqbkI_IxtV3z03S7LNTZtLVhFdrsEVc7F8MSPSTMwLshcfsfnkSNz-a9X2Yz8BZjNsO2TdfkooY24c3fHZOv56fP2Ws-f395m03nueXCdHkpEZUrC1uCsQIdmAU1zIEuLeiF0oJCLeSQO9SowQklQAtTLkyhHZfAx-T-2LuN4bvH1FWbJtlhAXgMfaoKUVAmCybUgN6doOvQRz-s21NCKCWMHCh2pGwMKUWsq21sNhB3FaPVQXL1L7k6SOa_IepyUQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2424466495</pqid></control><display><type>article</type><title>Activity of Alcohol Dehydrogenase and Aldehyde Dehydrogenase in Lung Cancer Cells</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>ORYWAL, KAROLINA ; JELSKI, WOJCIECH ; KOZŁOWSKI, MIROSŁAW DARIUSZ ; MROCZKO, BARBARA</creator><creatorcontrib>ORYWAL, KAROLINA ; JELSKI, WOJCIECH ; KOZŁOWSKI, MIROSŁAW DARIUSZ ; MROCZKO, BARBARA</creatorcontrib><description>Background: The aim of this study was to define the alterations in the activity of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in normal and cancerous lung cells. Materials and Methods: Lung tissues were taken from 36 patients during surgical resection of cancer. The activities of tested enzymes were measured by spectrofluorometric method (ADH I, ADH II, total ALDH) and photometric method (ADH III, ADH IV, total ADH). Results: The activities of class II and III ADH were significantly lower in lung cancer cells compared to histologically normal lung tissue. Conclusion: Reduced activity of isoenzyme class II ADH may affect disorders in retinoic acid biosynthesis, leading to its deficit. Lower ADH III activity may result in depletion of glutathione, and in initiation of oxidative stress, leading to cancer progression. These data suggest that alterations in ADH isoenzyme activities can contribute to carcinogenesis in human lungs.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>DOI: 10.21873/anticanres.14375</identifier><language>eng</language><publisher>Athens: International Institute of Anticancer Research</publisher><subject>Alcohol dehydrogenase ; Aldehyde dehydrogenase ; Aldehydes ; Biosynthesis ; Carcinogenesis ; Carcinogens ; Dehydrogenase ; Dehydrogenases ; Depletion ; Glutathione ; Isoenzymes ; Lung cancer ; Oxidative stress ; Retinoic acid</subject><ispartof>Anticancer research, 2020-07, Vol.40 (7), p.3857-3863</ispartof><rights>Copyright International Institute of Anticancer Research Jul 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-85ee6d82c8a9c4eda9b091da78ca7b6740af4582cde7e7ad464a7498b927d35a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>ORYWAL, KAROLINA</creatorcontrib><creatorcontrib>JELSKI, WOJCIECH</creatorcontrib><creatorcontrib>KOZŁOWSKI, MIROSŁAW DARIUSZ</creatorcontrib><creatorcontrib>MROCZKO, BARBARA</creatorcontrib><title>Activity of Alcohol Dehydrogenase and Aldehyde Dehydrogenase in Lung Cancer Cells</title><title>Anticancer research</title><description>Background: The aim of this study was to define the alterations in the activity of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in normal and cancerous lung cells. Materials and Methods: Lung tissues were taken from 36 patients during surgical resection of cancer. The activities of tested enzymes were measured by spectrofluorometric method (ADH I, ADH II, total ALDH) and photometric method (ADH III, ADH IV, total ADH). Results: The activities of class II and III ADH were significantly lower in lung cancer cells compared to histologically normal lung tissue. Conclusion: Reduced activity of isoenzyme class II ADH may affect disorders in retinoic acid biosynthesis, leading to its deficit. Lower ADH III activity may result in depletion of glutathione, and in initiation of oxidative stress, leading to cancer progression. These data suggest that alterations in ADH isoenzyme activities can contribute to carcinogenesis in human lungs.</description><subject>Alcohol dehydrogenase</subject><subject>Aldehyde dehydrogenase</subject><subject>Aldehydes</subject><subject>Biosynthesis</subject><subject>Carcinogenesis</subject><subject>Carcinogens</subject><subject>Dehydrogenase</subject><subject>Dehydrogenases</subject><subject>Depletion</subject><subject>Glutathione</subject><subject>Isoenzymes</subject><subject>Lung cancer</subject><subject>Oxidative stress</subject><subject>Retinoic acid</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpdkEtLAzEUhYMoWKs_wN2AGzdTk8lrsiz1CQURdD3cJnfaKdOkJjNC_73TVhC6unDPx-HwEXLL6KRgpeYP4LvGgo-YJkxwLc_IiGnDci05PScjWkiaa0rlJblKaU2pUqbkI_IxtV3z03S7LNTZtLVhFdrsEVc7F8MSPSTMwLshcfsfnkSNz-a9X2Yz8BZjNsO2TdfkooY24c3fHZOv56fP2Ws-f395m03nueXCdHkpEZUrC1uCsQIdmAU1zIEuLeiF0oJCLeSQO9SowQklQAtTLkyhHZfAx-T-2LuN4bvH1FWbJtlhAXgMfaoKUVAmCybUgN6doOvQRz-s21NCKCWMHCh2pGwMKUWsq21sNhB3FaPVQXL1L7k6SOa_IepyUQ</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>ORYWAL, KAROLINA</creator><creator>JELSKI, WOJCIECH</creator><creator>KOZŁOWSKI, MIROSŁAW DARIUSZ</creator><creator>MROCZKO, BARBARA</creator><general>International Institute of Anticancer Research</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20200701</creationdate><title>Activity of Alcohol Dehydrogenase and Aldehyde Dehydrogenase in Lung Cancer Cells</title><author>ORYWAL, KAROLINA ; JELSKI, WOJCIECH ; KOZŁOWSKI, MIROSŁAW DARIUSZ ; MROCZKO, BARBARA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-85ee6d82c8a9c4eda9b091da78ca7b6740af4582cde7e7ad464a7498b927d35a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alcohol dehydrogenase</topic><topic>Aldehyde dehydrogenase</topic><topic>Aldehydes</topic><topic>Biosynthesis</topic><topic>Carcinogenesis</topic><topic>Carcinogens</topic><topic>Dehydrogenase</topic><topic>Dehydrogenases</topic><topic>Depletion</topic><topic>Glutathione</topic><topic>Isoenzymes</topic><topic>Lung cancer</topic><topic>Oxidative stress</topic><topic>Retinoic acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ORYWAL, KAROLINA</creatorcontrib><creatorcontrib>JELSKI, WOJCIECH</creatorcontrib><creatorcontrib>KOZŁOWSKI, MIROSŁAW DARIUSZ</creatorcontrib><creatorcontrib>MROCZKO, BARBARA</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ORYWAL, KAROLINA</au><au>JELSKI, WOJCIECH</au><au>KOZŁOWSKI, MIROSŁAW DARIUSZ</au><au>MROCZKO, BARBARA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activity of Alcohol Dehydrogenase and Aldehyde Dehydrogenase in Lung Cancer Cells</atitle><jtitle>Anticancer research</jtitle><date>2020-07-01</date><risdate>2020</risdate><volume>40</volume><issue>7</issue><spage>3857</spage><epage>3863</epage><pages>3857-3863</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Background: The aim of this study was to define the alterations in the activity of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in normal and cancerous lung cells. Materials and Methods: Lung tissues were taken from 36 patients during surgical resection of cancer. The activities of tested enzymes were measured by spectrofluorometric method (ADH I, ADH II, total ALDH) and photometric method (ADH III, ADH IV, total ADH). Results: The activities of class II and III ADH were significantly lower in lung cancer cells compared to histologically normal lung tissue. Conclusion: Reduced activity of isoenzyme class II ADH may affect disorders in retinoic acid biosynthesis, leading to its deficit. Lower ADH III activity may result in depletion of glutathione, and in initiation of oxidative stress, leading to cancer progression. These data suggest that alterations in ADH isoenzyme activities can contribute to carcinogenesis in human lungs.</abstract><cop>Athens</cop><pub>International Institute of Anticancer Research</pub><doi>10.21873/anticanres.14375</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0250-7005
ispartof Anticancer research, 2020-07, Vol.40 (7), p.3857-3863
issn 0250-7005
1791-7530
language eng
recordid cdi_proquest_miscellaneous_2420152146
source EZB-FREE-00999 freely available EZB journals
subjects Alcohol dehydrogenase
Aldehyde dehydrogenase
Aldehydes
Biosynthesis
Carcinogenesis
Carcinogens
Dehydrogenase
Dehydrogenases
Depletion
Glutathione
Isoenzymes
Lung cancer
Oxidative stress
Retinoic acid
title Activity of Alcohol Dehydrogenase and Aldehyde Dehydrogenase in Lung Cancer Cells
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-23T23%3A51%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Activity%20of%20Alcohol%20Dehydrogenase%20and%20Aldehyde%20Dehydrogenase%20in%20Lung%20Cancer%20Cells&rft.jtitle=Anticancer%20research&rft.au=ORYWAL,%20KAROLINA&rft.date=2020-07-01&rft.volume=40&rft.issue=7&rft.spage=3857&rft.epage=3863&rft.pages=3857-3863&rft.issn=0250-7005&rft.eissn=1791-7530&rft_id=info:doi/10.21873/anticanres.14375&rft_dat=%3Cproquest_cross%3E2420152146%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c349t-85ee6d82c8a9c4eda9b091da78ca7b6740af4582cde7e7ad464a7498b927d35a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2424466495&rft_id=info:pmid/&rfr_iscdi=true