The expression level of cannabinoid receptors type 1 and 2 in the different types of astrocytomas

Astrocytomas, the most prevalent primary brain tumors, can be divided by histology and malignancy levels into four following types: pilocytic astrocytoma (grade I), diffuse fibrillary astrocytoma (grade II), anaplastic astrocytoma (grade III), and glioblastoma multiforme (grade IV). For high grade a...

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Bibliographic Details
Published in:Molecular biology reports 2020-07, Vol.47 (7), p.5461-5467
Main Authors: Hashemi, Mansoureh, Bashi, Senada, Zali, Alireza
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Astrocytoma
Biomedical and Life Sciences
Blood vessels
Brain cancer
Brain tumors
Cannabinoid CB1 receptors
Cannabinoid CB2 receptors
Glioblastoma
Glioblastoma multiforme
Histology
Immunofluorescence
Life Sciences
Malignancy
Morphology
Neurosurgery
Original Article
Polymerase chain reaction
Receptor mechanisms
Western blotting
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Summary:Astrocytomas, the most prevalent primary brain tumors, can be divided by histology and malignancy levels into four following types: pilocytic astrocytoma (grade I), diffuse fibrillary astrocytoma (grade II), anaplastic astrocytoma (grade III), and glioblastoma multiforme (grade IV). For high grade astrocytomas (grade III and grade IV), blood vessels formation is considered as the most important property. The distribution of cannabinoid receptors type 1 (CB1) and cannabinoid receptor type 2 (CB2) in blood vessels and tumor tissue of astrocytoma is still controversial. Asrocytoma tissues were collected from 45 patients under the condition of tumor-related neurosurgical operation. The expression of CB1 and CB2 receptors was assessed using immunofluorescence, quantitative real-time RT-PCR and western blotting. The results indicated an increased expression of CB1 receptors in tumor tissue. There was a significant difference in the mount of CB2 receptors in blood vessels. More was observed in the grade III and glioblastoma (grade IV) than astrocytoma of grade II and control. This study suggested that, the expression increase of cannabinoid receptors is an index for astrocytoma malignancy and can be targeted as a therapeutic approach for the inhibition of astrocytoma growth among patients.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-020-05636-8