Characterization of natural antigen-specific antibodies from naïve sturgeon serum

In this study, we isolated and characterized natural antibodies found in serum samples from Bester sturgeon (Huso huso × Acipenser ruthenus). Natural antibodies specifically detected hen egg lysozyme (HEL), keyhole limpet hemocyanin (KLH), and several species of pathogenic bacteria. Interestingly, w...

Full description

Saved in:
Bibliographic Details
Published in:Developmental and comparative immunology 2020-11, Vol.112, p.103770-103770, Article 103770
Main Authors: Yasumoto, Kyutaro, Koiwai, Keiichiro, Hiraoka, Kiyoshi, Hirono, Ikuo, Kondo, Hidehiro
Format: Article
Language:English
Subjects:
Affinity chromatography
Antibodies
Antibody
Antigens
Hatching
Immunoprecipitation
Lysozyme
Oncorhynchus mykiss
Salmon
Sturgeon
Trout
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, we isolated and characterized natural antibodies found in serum samples from Bester sturgeon (Huso huso × Acipenser ruthenus). Natural antibodies specifically detected hen egg lysozyme (HEL), keyhole limpet hemocyanin (KLH), and several species of pathogenic bacteria. Interestingly, we detected no antibodies with similar specificity in serum samples from rainbow trout (Oncorhynchus mykiss) or from Japanese flounder (Paralichthys olivaceus). Binding capacity of the sturgeon natural serum antibodies increased slightly at 7 months compared to 3 months after hatching. Antigen-specific antibodies against KLH, Aeromonas hydrophila and Streptococcus iniae were affinity-fractionated from naive sera of Bester sturgeon; specific detection of the corresponding antigens was observed. We conclude that Bester sturgeon are capable of generating unique natural antibodies including those that are pathogen-specific. •The sturgeon natural serum antibodies bound to various antigens.•The binding activities were increased from 3 to 7 months after hatching.•The antigen specific natural antibodies were fractionated and evaluated.
ISSN:0145-305X
1879-0089
DOI:10.1016/j.dci.2020.103770