Photoelectrochemical aptasensor for thrombin based on Au-rGO-CuS as signal amplification elements

A photoelectrochemical platform for thrombin determination was developed based on Au-rGO-CuS as multiple signal amplification elements. CuInS 2 QDs was used to sensitize burr-shape TiO 2 (b-TiO 2 ) to obtain a strong photocurrent. Under the specific recognition between aptamer and thrombin, a sandwi...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2020-08, Vol.187 (8), p.433-433, Article 433
Main Authors: Zou, Lina, Yang, Lingxi, Zhan, Yi, Huang, Di, Ye, Baoxian
Format: Article
Language:English
Subjects:
Amplification
Analytical Chemistry
Characterization and Evaluation of Materials
Charge transfer
Chemistry
Chemistry and Materials Science
Copper sulfides
Electron recombination
Microengineering
Nanochemistry
Nanotechnology
Original Paper
Photoelectric effect
Photoelectric emission
Steric hindrance
Thrombin
Titanium dioxide
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Summary:A photoelectrochemical platform for thrombin determination was developed based on Au-rGO-CuS as multiple signal amplification elements. CuInS 2 QDs was used to sensitize burr-shape TiO 2 (b-TiO 2 ) to obtain a strong photocurrent. Under the specific recognition between aptamer and thrombin, a sandwichlike structure was formed and the Au-rGO-CuS-labeled aptamer (S2@Au-rGO-CuS) was immobilized on the electrode surface. This induced a sharp decrease in photocurrent. The phenomenon is mainly due to the fact that CuS NPs can competitively consume the light energy and electron donor with CuInS 2 /b-TiO 2 . The rGO can increase the amount of CuS NPs and the Au NPs can accelerate charge transferring which depress the recombination of photogenerated electrons and holes in CuS to further enhance the competitive capacity of CuS. The sandwichlike structure has a steric hindrance effect. Therefore, the S2@Au-rGO-CuS has a multiple signal amplification function for thrombin determination. Under optimal conditions, the PEC aptasensor exhibited a wide linear concentration range from 0.1 pM to 10 nM with a low detection limit of 30 fM (S/ N  = 3) for thrombin. Besides, the designed aptasensor performed well in the assay of human serum sample, indicating good potential for the determination of thrombin in real samples. Graphical abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-020-04380-x