Maternal ageing causes changes in DNA methylation and gene expression profiles in mouse oocytes

Although it is well known that maternal ageing causes reduced oocyte quality and fertility, little information is known about its effect on germ cell epigenetics. In the present study, we compared the gene expression and DNA methylation profiles in germinal vesicle oocytes from young (8-week-old) an...

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Bibliographic Details
Published in:Zygote (Cambridge) 2020-10, Vol.28 (5), p.360-366
Main Authors: Hou, Guanmei, Sun, Qing-Yuan
Format: Article
Language:English
Subjects:
Aging
Chromosomes
Deoxyribonucleic acid
DNA
DNA methylation
DNA sequencing
Epigenetics
Fertility
Gametocytes
Gene expression
Genes
Genomes
Infertility
Mitochondria
Oocytes
Ribonucleic acid
RNA
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Summary:Although it is well known that maternal ageing causes reduced oocyte quality and fertility, little information is known about its effect on germ cell epigenetics. In the present study, we compared the gene expression and DNA methylation profiles in germinal vesicle oocytes from young (8-week-old) and aged (18-month-old) mice using single-cell RNA-sequencing and single-cell whole-genome DNA methylation sequencing. We found significant differences in the data from the two groups. Oocytes from aged mice showed significant changes in the expression of some metabolism-related genes, such as mitochondria-associated genes, that was in line with our expectations. Expression of some genes associated with reproduction also showed significant differences. DNA methylation levels were also changed in oocytes from aged mice. The two groups had significant gaps in hypermethylation and hypomethylation levels on each chromosome. These data provide useful information for further understanding the mechanisms of oocyte ageing.
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199420000143