RNA interference of eclosion hormone gene reveals its roles in the control of ecdysis behavior in Heortia vitessoides Moore

Eclosion hormone (EH) is an important neuropeptide that regulates growth and development. This study predicted the EH gene (HvEH) of Heortia vitessoides Moore (Lepidoptera: Crambidae) from the transcriptome database and its expression patterns were determined using quantitative real‐time polymerase...

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Bibliographic Details
Published in:Archives of insect biochemistry and physiology 2020-09, Vol.105 (1), p.e21726-n/a
Main Authors: Li, Zhixing, Ye, Qingya, Lyu, Zihao, Lin, Tong
Format: Article
Language:English
Subjects:
20‐hydroxyecdysone
Developmental stages
ecdysis
Eclosion hormone
EH gene
Extreme values
Gene expression
Heortia vitessoides
Injection
Interference
Molting
Phenotypes
Polymerase chain reaction
Pupae
Pupation
Ribonucleic acid
RNA
RNA interference
RNA-mediated interference
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Summary:Eclosion hormone (EH) is an important neuropeptide that regulates growth and development. This study predicted the EH gene (HvEH) of Heortia vitessoides Moore (Lepidoptera: Crambidae) from the transcriptome database and its expression patterns were determined using quantitative real‐time polymerase chain reaction. HvEH was expressed in all developmental stages and especially in the head area. RNA interference‐mediated silencing of HvEH (2 μg/individual) with double‐stranded HvEH RNA (dsHvEH) was achieved within 48 hr. Abnormal phenotypes appeared in the pupa and adult stages. dsHvEH injection suppressed pupation and eclosion rates. HvEH expression increased upon treatment with 20‐hydroxyecdysone but decreased at extreme temperatures. These results suggest that HvEH plays an essential role in ecdysis and wing formation in H. vitessoides. Relative HvEH transcript levels in L5D1 larvae after injection with dsHvEH at concentrations of 2, 3, and 4 μg/μl for 24, 48, 72, and 96 hr, respectively, showed that RNA interference has a good effect under the condition of appropriate concentration and time. When injected with dsHvEH at 2 μg/μl in subsequent experiments, malformed phenotypes appeared in the pupal and adult stages. Research Highlights 1. RNA interference was used to silence the eclosion hormone gene and verify it functions; 2. The injection of exogenous 20‐hydroxyecdysone was attempted to verify its effect; 3. Temperature also has a certain influence on the expression of eclosion hormone.
ISSN:0739-4462
1520-6327
DOI:10.1002/arch.21726