The secondary metabolite regulator, BbSmr1, is a central regulator of conidiation via the BrlA‐AbaA‐WetA pathway in Beauveria bassiana

• Published in: Environmental microbiology 2021-02, Vol.23 (2), p.810-825
• Main Authors: Chen, Jin‐Feng, Liu, Yu, Tang, Gui‐Rong, Jin, Dan, Chen, Xi, Pei, Yan, Fan, Yan‐Hua
• Format: Article
• Language: English
• Subjects: Animals; Beauveria - genetics; Beauveria bassiana; Biological control; Biological Control Agents - metabolism; Cell Wall - metabolism; Cell walls; Clonal deletion; Conidia; Entomopathogenic fungi; Epitopes; Fungal Proteins - genetics; Fungi; Gene Expression Regulation, Fungal - genetics; Germination; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Insecta - microbiology; Insects; Metabolites; Nucleotide sequence; Pathogens; Pest control; Phenotypes; Promoter Regions, Genetic - genetics; Promoters; Propagules; Regulatory mechanisms (biology); Reproduction, Asexual - physiology; Spores, Fungal - cytology; Spores, Fungal - metabolism; Transcription; Transcription, Genetic - genetics; Yields
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/1462-2920.15155

Summary The filamentous fungus Beauveria bassiana, an insect fungal pathogen, is widely used for pest biocontrol. Aerial conidia are infectious propagules, and their yield and viability greatly affect the field application of this fungus; however, little is known about the molecular regulatory mechanism of the triggered conidiation. In the present study, we find that the secondary metabolite regulator BbSmr1 is involved in the regulation of asexual conidiation development and stress response in B. bassiana. A deficiency in Bbsmr1 results in a prominent fluffy‐like phenotype on solid medium, decreased conidial yield, accelerated conidial germination, as well as increased tolerance to H2O2 stress and cell wall inhibitors. The deletion of Bbsmr1 also leads to thickened conidial cell walls and changed cell epitopes. Overexpressing either BbbrlA or BbabaA in the ∆Bbsmr1 strain can rescue the phenotypes of conidial development and stress response. BbSmr1 activates BbbrlA transcription by directly binding to the A4GA3 sequence of the BbbrlA promoter. BbBrlA in turn binds to the promoter of Bbsmr1 and negatively regulates the expression of Bbsmr1. These results indicate that BbSmr1 positively regulates conidial development in B. bassiana by activating the central development pathway BrlA‐AbaA‐WetA and provides insights into the developmental regulatory mechanism of entomopathogenic fungi.