Multifunctional solvothermal carbon derived from alginate using 'water-in-deep eutectic solvents' for enhancing enzyme activity

Herein, the use of a "water-in-deep eutectic solvent (DES)" system has been shown as an alternative platform for the low temperature conversion of alginic acid (AA) to a multifunctional aliginate derived carbon (AAC) material with variable oxygen functionalities. Using the in-built oxygena...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2020-08, Vol.56 (67), p.9659-9662
Main Authors: Yadav, Niketa, Bisht, Meena, Nataraj, S. K, Venkatesu, Pannuru, Mondal, Dibyendu
Format: Article
Language:English
Subjects:
Alginates
Alginates - chemistry
Carbon
Carbon - chemistry
Cytochromes
Cytochromes c - chemistry
Cytochromes c - metabolism
Enzyme activity
Enzymes
Immobilized Proteins - chemistry
Immobilized Proteins - metabolism
Low temperature
Peroxidase
Peroxidases - metabolism
Proteins
Solvents
Structural stability
Water - chemistry
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Summary:Herein, the use of a "water-in-deep eutectic solvent (DES)" system has been shown as an alternative platform for the low temperature conversion of alginic acid (AA) to a multifunctional aliginate derived carbon (AAC) material with variable oxygen functionalities. Using the in-built oxygenated functionalities in AAC, cytochrome- c (Cyt- c ) was immobilised on the surface of AACs and the peroxidase activity of the enzyme was studied. Remarkably, the enzymatic activity was enhanced up to 5.5-fold compared to the native protein without compromising the structural stability of Cyt- c . Altogether, the present study demonstrates a sustainable process for the preparation of AACs and shows their beneficial effect on the activity and stability of Cyt- c , and thus, their application as a protein-friendly material for biotechnological applications. The use of a water-in-DES system for conversion of a seaweed biopolymer to a highly oxygenated functional carbon is reported for protein packaging with improved activity and stability.
ISSN:1359-7345
1364-548X
DOI:10.1039/d0cc03866k