Lignolytic and hemicellulolytic enzyme cocktail production from Bacillus tequilensis LXM 55 and its application in pulp biobleaching

• Published in: Bioprocess and biosystems engineering 2020-12, Vol.43 (12), p.2219-2229
• Main Authors: Angural, Steffy, Kumar, Aditya, Kumar, Deepak, Warmoota, Rahul, Sondhi, Sonica, Gupta, Naveen
• Format: Article
• Language: English
• Subjects: Bacillus tequilensis; Bioprocessing; Biotechnology; Chemistry; Chemistry and Materials Science; Chlorine; Environmental Engineering/Biotechnology; Enzymes; Food Science; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Laccase; Mannanases; Optimization; Pulp; Pulp & paper mills; Research Paper; Xylanase
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-020-02407-4

Bioprocessing of pulp requires lignolytic as well as hemicellulolytic enzymes. The present study is the first report of a cocktail of laccase (L), xylanase (X), and mannanase (M), from a single bacterium for pulp biobleaching. A novel strain Bacillus tequilensis LXM 55 produced thermo-alkali stable L + X + M. On optimization higher enzyme yield (IUml −1 /fold increase) of laccase (396.35/24.16), xylanase (212.95/81.90) and mannanase (153.33/102.90) were achieved in the cocktail. Treatment of pulp with cocktail of enzymes led to 49.35% reduction in kappa number and considerable enhancement in the brightness (11.59%), whiteness (4.11%), and other pulp properties. Most importantly, no mediator system was required for the application of laccase. 40% less chlorine consumption was required to obtain the paper of the same quality as that of pulp treated without enzyme but with 100% chlorine. Therefore, this cocktail of enzymes is highly suitable for pulp biobleaching in the paper mill.