Expression pattern of GLUT 1, 5, 8 and citrate synthase transcripts in buffalo (Bubalus bubalis) preimplantation embryos produced in vitro and derived in vivo

In vitro‐produced (IVP) embryos are reported to be developmentally lesser competent than in vivo‐derived (IVD) embryos and supposed to differ in the expression of genes related with glucose metabolism. So, the present study was conducted to analyse the expression pattern of GLUT 1, 5, 8 and citrate...

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Bibliographic Details
Published in:Reproduction in domestic animals 2020-10, Vol.55 (10), p.1362-1370
Main Authors: Taru Sharma, Gutulla, Nath, Amar, Prasad, Shiv, Singhal, Sumit, Chandra, Vikash, Saikumar, Gutulla
Format: Article
Language:English
Subjects:
Abattoirs
Blastocysts
Buffalo
Cell culture
Citrate synthase
developmental competence
Developmental stages
Embryos
Gametocytes
Gene expression
Genes
Glucose metabolism
Glucose transporter type 5
GLUTs
In vitro fertilization
Kinases
Oocytes
Pattern analysis
preimplantation embryos
Ribonucleic acid
RNA
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Summary:In vitro‐produced (IVP) embryos are reported to be developmentally lesser competent than in vivo‐derived (IVD) embryos and supposed to differ in the expression of genes related with glucose metabolism. So, the present study was conducted to analyse the expression pattern of GLUT 1, 5, 8 and citrate synthase (CS) in oocytes and embryos produced in vivo or in vitro in buffalo. IVD embryos were obtained from 18 superovulated buffaloes. IVP embryos were obtained from slaughterhouse‐derived oocytes subsequently subjected to in vitro fertilization and culture. Total RNA was isolated from different stages of oocytes (immature and in vitro matured) and embryos (8–16 cell to blastocysts of IVP embryos and morula to blastocysts of IVD embryos). Results demonstrated that the expression of GLUT1, GLUT 8 increased from 8 to 16 cells to blastocyst and was significantly (p < .05) higher in IVP embryos. Expression of both genes was (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13782