Herbivore‐induced DMNT catalyzed by CYP82D47 plays an important role in the induction of JA‐dependent herbivore resistance of neighboring tea plants

• Published in: Plant, cell and environment cell and environment, 2021-04, Vol.44 (4), p.1178-1191
• Main Authors: Jing, Tingting, Du, Wenkai, Gao, Ting, Wu, Yi, Zhang, Na, Zhao, Mingyue, Jin, Jieyang, Wang, Jingming, Schwab, Wilfried, Wan, Xiaochun, Song, Chuankui
• Format: Article
• Language: English
• Subjects: Accumulation; Alkenes - metabolism; Allelochemicals; Animals; Biosynthesis; Camellia sinensis - genetics; Camellia sinensis - metabolism; Camellia sinensis - physiology; Cloning, Molecular; Communication; CsCYP82D47; Cyclopentanes - metabolism; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; Damage accumulation; Gene Expression Regulation, Plant; Herbivores; herbivorous insects; HIPVs; Insects; JA pathway; Jasmonic acid; Larva; Moths; Nerolidol; Oxylipins - metabolism; Pest resistance; Pests; Plant Defense Against Herbivory; Plant Growth Regulators - metabolism; Plant Growth Regulators - physiology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant resistance; plant–plant communication; Real-Time Polymerase Chain Reaction; Risk reduction; Sequence Analysis, DNA; Tea; tea plant; Volatile compounds; Volatile Organic Compounds - metabolism; Volatiles
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/pce.13861

Herbivore‐induced plant volatiles play important ecological roles in defense against stresses. However, if and which volatile(s) are involved in the plant–plant communication in response to herbivorous insects in tea plants remains unknown. Here, plant–plant communication experiments confirm that volatiles emitted from insects‐attacked tea plants can trigger plant resistance and reduce the risk of herbivore damage by inducing jasmonic acid (JA) accumulation in neighboring plants. The emission of six compounds was significantly induced by geometrid Ectropis obliqua, one of the most common pests of the tea plant in China. Among them, (E)‐4,8‐dimethyl‐1,3,7‐nonatriene (DMNT) could induce the accumulation of JA and thus promotes the resistance of neighboring intact plants to herbivorous insects. CsCYP82D47 was identified for the first time as a P450 enzyme, which catalyzes the final step in the biosynthesis of DMNT from (E)‐nerolidol. Down‐regulation of CsCYP82D47 in tea plants resulted in a reduced accumulation of DMNT and significantly reduced the release of DMNT in response to the feeding of herbivorous insects. The first evidence for plant–plant communication in response to herbivores in tea plants will help to understand how plants respond to volatile cues in response to herbivores and provide new insight into the role(s) of DMNT in tea plants. Plant–plant communication is of great importance as it allows plants and the partners they interact with to tune their growth and defense. However, if and which volatile(s) are involved in the plant–plant communication in response to herbivorous insects in tea plants remains unknown. Here, we provide the first evidence for plant–plant communication via inducible volatiles in response to herbivores damage in tea plants, and CsCYP82D47 was identified for the first time as a P450 enzyme involved in the formation of herbivore‐induced DMNT, which plays a key role(s) in priming plant defense against herbivores of their neighbors. The knowledge will help to understand how plants respond to volatile cues in response to herbivores and provide new insight into the role(s) and formation of DMNT in tea plants.