The interference of alpha- and beta-naphthoflavone with triclosan effects on viability, apoptosis and reactive oxygen species production in mouse neocortical neurons

• Published in: Pesticide biochemistry and physiology 2020-09, Vol.168, p.104638-104638, Article 104638
• Main Authors: Szychowski, Konrad A., Rybczyńska-Tkaczyk, Kamila, Gmiński, Jan, Wójtowicz, Anna K.
• Format: Article
• Language: English
• Subjects: Alpha-naphthoflavone; Beta-naphthoflavone; Neuron; ROS; Triclosan; Xanthine oxidase
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2020.104638

[Display omitted] •αNF and βNF enhance the TCS-stimulated effects on LDH release and caspase-3 activity.•αNF and βNF attenuate TCS-stimulated effects on ROS.•αNF inhibits XO activity more strongly than quercetin. Triclosan (TCS) is commonly used worldwide in a range of personal care and sanitizing products. A number of studies have revealed the presence of TCS in human tissues. It has recently been shown that TCS can interact with AhR in mouse neurons and the one of its effects is the stimulation of reactive oxygen species (ROS) production. Reactive oxygen species perform a wide spectrum of functions in neuronal cells, where they are generated as by-products of cellular metabolism. Therefore the aim of the study was to investigate effects of two synthetic naphthoflavones, the beta-naphthoflavone (βNF) and alpha-naphthoflavone (αNF), well known agonist and antagonist of AhR on TCS-stimulated cytotoxicity, apoptosis and ROS production in mouse primary cortical neurons in vitro cultures. The results showed that both agonist (βNF) and antagonist (αNF) of AhR enhanced the LDH release and caspase-3 activity stimulated by TCS. Interestingly, both naphthoflavones decreased the TCS-stimulated ROS production, however, they showed no scavenging properties as revealed by ABTS•+ and DPPH• methods. What's more, both βNF as well as αNF inhibited the activity of xanthine oxidase (XO) stimulated by TCS. Thus, we can assume that αNF or βNF act in a competitive way over TCS and inhibit its effect on antioxidant enzyme activity.