Demonstration of the cryoprotective properties of the fucose-containing polysaccharide FucoPol

[Display omitted] •FucoPol increased post-thaw cell viability of multiple cell lines.•Good cryoprotective effect was achieved at a low concentration of 2.5 mg/mL.•FucoPol showed no cytotoxicity and cell morphology was preserved.•FucoPol showed calorimetric behavioural similarities to an antifreeze p...

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Bibliographic Details
Published in:Carbohydrate polymers 2020-10, Vol.245, p.116500-116500, Article 116500
Main Authors: Guerreiro, Bruno M., Freitas, Filomena, Lima, João C., Silva, Jorge C., Dionísio, Madalena, Reis, Maria A.M.
Format: Article
Language:English
Subjects:
Animals
Antifreeze Proteins - chemistry
Calorimetry
Calorimetry, Differential Scanning
Cell Line, Tumor
Cell Survival - drug effects
Chlorocebus aethiops
Cryopreservation
Cryopreservation - methods
Cryoprotective Agents - chemistry
Cryoprotective Agents - pharmacology
Crystallization
Cytotoxicity
Enterobacter - chemistry
Freezing
FucoPol
Fucose - chemistry
Fucose - pharmacology
Humans
Ice
Mice
Non-colligative
Polyelectrolytes - chemistry
Polysaccharide
Polysaccharides, Bacterial - chemistry
Polysaccharides, Bacterial - pharmacology
Vero Cells
Water - chemistry
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Summary:[Display omitted] •FucoPol increased post-thaw cell viability of multiple cell lines.•Good cryoprotective effect was achieved at a low concentration of 2.5 mg/mL.•FucoPol showed no cytotoxicity and cell morphology was preserved.•FucoPol showed calorimetric behavioural similarities to an antifreeze protein.•FucoPol induced premature crystal growth, thus promoting reduced crystal sizes. We report the cryoprotective potential of FucoPol, a fucose-containing bacterial exopolysaccharide produced by Enterobacter A47. In vitro cryopreservation assays of Vero, Saos-2, HFFF2 and C2C12 cell lines exposed to a validated non-cytotoxic 2.5 mg/mL FucoPol concentration demonstrated a consistent post-thaw metabolic viability increase. Calorimetric analysis showed a non-colligative, FucoPol concentration-dependent increase of the freezing point (Tf), with minimal change in melting point (Tm). Freezing point variation was corroborated by Polarized Optical Microscopy studies, also showing a reduction of ice crystal dimensions. Its proven shear-thinning behaviour and polyanionicity favour interactivity between the polysaccharide and the water-ice interface, resulting in ice growth inhibition. These findings demonstrate FucoPol’s high promise as a bio-based, biodegradable approach to be implemented into cryopreservation formulations.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2020.116500