A study on the nitrogen removal efficacy of bacterium Acinetobacter tandoii MZ-5 from a contaminated river of Shenzhen, Guangdong Province, China

• Published in: Bioresource technology 2020-11, Vol.315, p.123888-123888, Article 123888
• Main Authors: Ouyang, Liao, Wang, Keju, Liu, Xinyue, Wong, Ming Hung, Hu, Zhangli, Chen, Huirong, Yang, Xuewei, Li, Shuangfei
• Format: Article
• Language: English
• Subjects: Heterotrophic nitrification-aerobic denitrification; Nitrite removal; River sediment; Wastewater treatment
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2020.123888

[Display omitted] •Strain MZ-5 showed heterotrophic nitrification and aerobic nitrification capability.•Ammonium was the priority nitrogen source in the mixed N sources.•napA gene was successfully amplified.•Strain MZ-5 showed good nitrogen removal performance when treating wastewater. Heterotrophic nitrification–aerobic denitrification (HN-AD) has advantages over the traditional nitrogen removal process when removing multiple types of nitrogen in wastewater treatment. Acinetobacter tandoii MZ-5, which is capable of HN-AD, was isolated from the sediment of a polluted river for the first time. It used NH4+-N, NO2–-N and NO3–-N as sole nitrogen sources with maximum removal rates of 2.28, 1.18 and 1.04 mg L−1h−1, respectively. Simultaneous nitrification and denitrification were observed when using mixed N sources and NH4+-N was preferentially utilized. High nitrogen removal efficiencies (>90%) were achieved under the following conditions: C/N ratio 11–18, pH 6–8, 25–30 °C and dissolved oxygen 7.35–7.66 mg L−1. Strain MZ-5 was effective at treating wastewater from landfill leachate treatment plants, with NH4+-N, NO3–-N and total nitrogen (TN) removal efficiencies of 99.28%, 44.85% and 45.31%, respectively. Thus, strain MZ-5 may be a good candidate for wastewater treatment.