Fully-printed electrochemical sensors made with flexible screen-printed electrodes modified by roll-to-roll slot-die coating

• Published in: Biosensors & bioelectronics 2020-10, Vol.165, p.112428-112428, Article 112428
• Main Authors: Cagnani, Giovana Rosso, Ibáñez-Redín, Gisela, Tirich, Beatriz, Gonçalves, Débora, Balogh, Debora T., Oliveira, Osvaldo N.
• Format: Article
• Language: English
• Subjects: Biosensing Techniques; Carbon; Carbon black; Catechol; Dopamine; Electrochemical sensors; Electrodes; Ink; Printed biosensors; Reproducibility of Results; Roll-to-roll; Slot-die deposition
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2020.112428

The manufacture of sensors using large-scale production techniques, such as roll-to-roll (R2R) processing, may fulfill requirements of low-cost disposable devices. Herein, we report the fabrication of fully-printed electrochemical sensors using screen-printed carbon electrodes coated with carbon black inks through slot-die coating within an R2R process. As a proof of concept, sensors were produced to detect the neurotransmitter dopamine with high reproducibility and low limit of detection (0.09 μmol L−1). Furthermore, fully-printed biosensors made with a tyrosinase-containing ink were used to detect catechol in natural water samples. Since slot-die deposition enables printing enzymes without significant activity loss, the biosensors exhibited high stability over a period of several weeks. Even more important, R2R slot-die coating may be extended to any type of sensors and biosensors with the possibility of large-scale manufacturing. [Display omitted] •Fabrication of large-scale printed electrochemical sensors.•Novel methodology for fabrication of fully-printed disposable sensors and biosensors using roll-to-roll processing.•Fabrication of screen-printed carbon electrodes modified with carbon black and enzymatic inks using slot-die deposition.•Fabrication of devices applied to dopamine and catechol detection.