Effect of root surface treatment with denusomab after delayed tooth replantation

Objectives To evaluate denosumab, a human monoclonal antibody that mimics the effects of osteoprotegerin in bone metabolism, as a topical treatment of root surface to be used prior to delayed tooth replantation. Materials and methods Thirty-six rats’ right incisors were used. Teeth were extracted an...

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Bibliographic Details
Published in:Clinical oral investigations 2021-03, Vol.25 (3), p.1255-1264
Main Authors: da Silva, Léa Assed Bezerra, Longo, Daniele Lucca, Stuani, Maria Bernadete Sasso, de Queiroz, Alexandra Mussolino, da Silva, Raquel Assed Bezerra, Nelson-Filho, Paulo, Vieira, Heloisa Aparecida Orsini, Pucinelli, Carolina Maschietto, Paula-Silva, Francisco Wanderley Garcia
Format: Article
Language:English
Subjects:
Animals
Ankylosis
Bone turnover
Calcium hydroxide
Contamination
Dental roots
Dentistry
Immunohistochemistry
Immunotherapy
Incisor
Incisors
Labeling
Medicine
Monoclonal antibodies
Original Article
Osteoprotegerin
Periodontal Ligament
Rats
Root canals
Root resorption
Root Resorption - prevention & control
Teeth
Tooth Ankylosis
Tooth Replantation
Tooth Root
TRANCE protein
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Summary:Objectives To evaluate denosumab, a human monoclonal antibody that mimics the effects of osteoprotegerin in bone metabolism, as a topical treatment of root surface to be used prior to delayed tooth replantation. Materials and methods Thirty-six rats’ right incisors were used. Teeth were extracted and divided into: delayed replantation without root surface treatment (control); delayed replantation with root surface treatment with denosumab 60 mg/mL and 30 mg/mL, respectively, for 10 min both experimentals groups. After that, the root canals were filled with calcium hydroxide and replanted. After 15 and 60 days, the animals were euthanized, and the samples were collected and processed for microscopic analysis. Histological sections were performed, and stained with HE to describe the dental characteristics, measure ankylosis, replacement resorption, and dental resorption by conventional microscopy. Also, was performed Brown & Brenn staining and immunohistochemistry for RANKL, OPG, and periostin. Results Denosumab 60 mg/mL reducted ankylosis ( p  
ISSN:1432-6981
1436-3771
DOI:10.1007/s00784-020-03430-1