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Effect of root surface treatment with denusomab after delayed tooth replantation
Objectives To evaluate denosumab, a human monoclonal antibody that mimics the effects of osteoprotegerin in bone metabolism, as a topical treatment of root surface to be used prior to delayed tooth replantation. Materials and methods Thirty-six rats’ right incisors were used. Teeth were extracted an...
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| Published in: | Clinical oral investigations 2021-03, Vol.25 (3), p.1255-1264 |
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| creator | da Silva, Léa Assed Bezerra Longo, Daniele Lucca Stuani, Maria Bernadete Sasso de Queiroz, Alexandra Mussolino da Silva, Raquel Assed Bezerra Nelson-Filho, Paulo Vieira, Heloisa Aparecida Orsini Pucinelli, Carolina Maschietto Paula-Silva, Francisco Wanderley Garcia |
| description | Objectives
To evaluate denosumab, a human monoclonal antibody that mimics the effects of osteoprotegerin in bone metabolism, as a topical treatment of root surface to be used prior to delayed tooth replantation.
Materials and methods
Thirty-six rats’ right incisors were used. Teeth were extracted and divided into: delayed replantation without root surface treatment (control); delayed replantation with root surface treatment with denosumab 60 mg/mL and 30 mg/mL, respectively, for 10 min both experimentals groups. After that, the root canals were filled with calcium hydroxide and replanted. After 15 and 60 days, the animals were euthanized, and the samples were collected and processed for microscopic analysis. Histological sections were performed, and stained with HE to describe the dental characteristics, measure ankylosis, replacement resorption, and dental resorption by conventional microscopy. Also, was performed Brown & Brenn staining and immunohistochemistry for RANKL, OPG, and periostin.
Results
Denosumab 60 mg/mL reducted ankylosis (
p
|
| doi_str_mv | 10.1007/s00784-020-03430-1 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2429060534</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2488187629</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-c49a8e90292617f3c07c49b7d6ed6c8337d8501c5b4a4553cc8a08f86bd044733</originalsourceid><addsrcrecordid>eNp9kEtPJSEQRolx4uOOf8CFIXHjpp3i0UAvjfGVmMwsZtaEpgu95nZzBTrGfy_j9ZG4cAMUnK-oHEIOGZwyAP0r18XIBjg0IKSAhm2RPSaFaoTWbPv1zBvVGbZL9nN-AGBSabFDdgXX3HQS9sifixDQFxoDTTEWmucUnEdaEroy4lTo07Lc0wGnOcfR9dSFgqnWK_eMAy01c08TrlduKq4s4_ST_AhulfHgbV-Qf5cXf8-vm9vfVzfnZ7eNF7otjZedM9gB77hiOggPul71elA4KG-E0INpgfm2l062rfDeODDBqH4AKbUQC3Ky6btO8XHGXOy4zB5XdRCMc7Zc8g4UtFXMghx_QR_inKY6XaWMYUYr3lWKbyifYs4Jg12n5ejSs2Vg__u2G9-2-ravvi2roaO31nM_4vAReRdcAbEBcn2a7jB9_v1N2xcpvYod</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2488187629</pqid></control><display><type>article</type><title>Effect of root surface treatment with denusomab after delayed tooth replantation</title><source>Springer Nature</source><creator>da Silva, Léa Assed Bezerra ; Longo, Daniele Lucca ; Stuani, Maria Bernadete Sasso ; de Queiroz, Alexandra Mussolino ; da Silva, Raquel Assed Bezerra ; Nelson-Filho, Paulo ; Vieira, Heloisa Aparecida Orsini ; Pucinelli, Carolina Maschietto ; Paula-Silva, Francisco Wanderley Garcia</creator><creatorcontrib>da Silva, Léa Assed Bezerra ; Longo, Daniele Lucca ; Stuani, Maria Bernadete Sasso ; de Queiroz, Alexandra Mussolino ; da Silva, Raquel Assed Bezerra ; Nelson-Filho, Paulo ; Vieira, Heloisa Aparecida Orsini ; Pucinelli, Carolina Maschietto ; Paula-Silva, Francisco Wanderley Garcia</creatorcontrib><description>Objectives
To evaluate denosumab, a human monoclonal antibody that mimics the effects of osteoprotegerin in bone metabolism, as a topical treatment of root surface to be used prior to delayed tooth replantation.
Materials and methods
Thirty-six rats’ right incisors were used. Teeth were extracted and divided into: delayed replantation without root surface treatment (control); delayed replantation with root surface treatment with denosumab 60 mg/mL and 30 mg/mL, respectively, for 10 min both experimentals groups. After that, the root canals were filled with calcium hydroxide and replanted. After 15 and 60 days, the animals were euthanized, and the samples were collected and processed for microscopic analysis. Histological sections were performed, and stained with HE to describe the dental characteristics, measure ankylosis, replacement resorption, and dental resorption by conventional microscopy. Also, was performed Brown & Brenn staining and immunohistochemistry for RANKL, OPG, and periostin.
Results
Denosumab 60 mg/mL reducted ankylosis (
p
< 0.0001), replacement resorption (
p
< 0.0001), and tooth resorption, 60 days after replantation, compared to untreated replanted teeth (
p
< 0.005). Lower bacterial contamination in root surface in the denosumab treatment groups was found, regardless of the concentration used (
p
< 0.001). Also, denosumab treatment inhibited the expression of RANKL without modulating OPG. Periostin was observed in periodontal ligament of replanted tooth, although this labelling was absent in the ankylosis areas, in both experimental periods.
Conclusion
Treatment of the root surface with denosumab at 60 mg/mL of rat teeth before delayed replantation reduced dental root resorption compared with the untreated teeth after 60 days.
Clinical relevance
Survival of a replanted tooth has been a challenge in clinical practice. The use of a medication, such as denosumab, to limit dental root resorption represents an important therapeutical approach.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-020-03430-1</identifier><identifier>PMID: 32728940</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Ankylosis ; Bone turnover ; Calcium hydroxide ; Contamination ; Dental roots ; Dentistry ; Immunohistochemistry ; Immunotherapy ; Incisor ; Incisors ; Labeling ; Medicine ; Monoclonal antibodies ; Original Article ; Osteoprotegerin ; Periodontal Ligament ; Rats ; Root canals ; Root resorption ; Root Resorption - prevention & control ; Teeth ; Tooth Ankylosis ; Tooth Replantation ; Tooth Root ; TRANCE protein</subject><ispartof>Clinical oral investigations, 2021-03, Vol.25 (3), p.1255-1264</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-c49a8e90292617f3c07c49b7d6ed6c8337d8501c5b4a4553cc8a08f86bd044733</citedby><cites>FETCH-LOGICAL-c375t-c49a8e90292617f3c07c49b7d6ed6c8337d8501c5b4a4553cc8a08f86bd044733</cites><orcidid>0000-0002-0230-1347</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32728940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Silva, Léa Assed Bezerra</creatorcontrib><creatorcontrib>Longo, Daniele Lucca</creatorcontrib><creatorcontrib>Stuani, Maria Bernadete Sasso</creatorcontrib><creatorcontrib>de Queiroz, Alexandra Mussolino</creatorcontrib><creatorcontrib>da Silva, Raquel Assed Bezerra</creatorcontrib><creatorcontrib>Nelson-Filho, Paulo</creatorcontrib><creatorcontrib>Vieira, Heloisa Aparecida Orsini</creatorcontrib><creatorcontrib>Pucinelli, Carolina Maschietto</creatorcontrib><creatorcontrib>Paula-Silva, Francisco Wanderley Garcia</creatorcontrib><title>Effect of root surface treatment with denusomab after delayed tooth replantation</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives
To evaluate denosumab, a human monoclonal antibody that mimics the effects of osteoprotegerin in bone metabolism, as a topical treatment of root surface to be used prior to delayed tooth replantation.
Materials and methods
Thirty-six rats’ right incisors were used. Teeth were extracted and divided into: delayed replantation without root surface treatment (control); delayed replantation with root surface treatment with denosumab 60 mg/mL and 30 mg/mL, respectively, for 10 min both experimentals groups. After that, the root canals were filled with calcium hydroxide and replanted. After 15 and 60 days, the animals were euthanized, and the samples were collected and processed for microscopic analysis. Histological sections were performed, and stained with HE to describe the dental characteristics, measure ankylosis, replacement resorption, and dental resorption by conventional microscopy. Also, was performed Brown & Brenn staining and immunohistochemistry for RANKL, OPG, and periostin.
Results
Denosumab 60 mg/mL reducted ankylosis (
p
< 0.0001), replacement resorption (
p
< 0.0001), and tooth resorption, 60 days after replantation, compared to untreated replanted teeth (
p
< 0.005). Lower bacterial contamination in root surface in the denosumab treatment groups was found, regardless of the concentration used (
p
< 0.001). Also, denosumab treatment inhibited the expression of RANKL without modulating OPG. Periostin was observed in periodontal ligament of replanted tooth, although this labelling was absent in the ankylosis areas, in both experimental periods.
Conclusion
Treatment of the root surface with denosumab at 60 mg/mL of rat teeth before delayed replantation reduced dental root resorption compared with the untreated teeth after 60 days.
Clinical relevance
Survival of a replanted tooth has been a challenge in clinical practice. The use of a medication, such as denosumab, to limit dental root resorption represents an important therapeutical approach.</description><subject>Animals</subject><subject>Ankylosis</subject><subject>Bone turnover</subject><subject>Calcium hydroxide</subject><subject>Contamination</subject><subject>Dental roots</subject><subject>Dentistry</subject><subject>Immunohistochemistry</subject><subject>Immunotherapy</subject><subject>Incisor</subject><subject>Incisors</subject><subject>Labeling</subject><subject>Medicine</subject><subject>Monoclonal antibodies</subject><subject>Original Article</subject><subject>Osteoprotegerin</subject><subject>Periodontal Ligament</subject><subject>Rats</subject><subject>Root canals</subject><subject>Root resorption</subject><subject>Root Resorption - prevention & control</subject><subject>Teeth</subject><subject>Tooth Ankylosis</subject><subject>Tooth Replantation</subject><subject>Tooth Root</subject><subject>TRANCE protein</subject><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kEtPJSEQRolx4uOOf8CFIXHjpp3i0UAvjfGVmMwsZtaEpgu95nZzBTrGfy_j9ZG4cAMUnK-oHEIOGZwyAP0r18XIBjg0IKSAhm2RPSaFaoTWbPv1zBvVGbZL9nN-AGBSabFDdgXX3HQS9sifixDQFxoDTTEWmucUnEdaEroy4lTo07Lc0wGnOcfR9dSFgqnWK_eMAy01c08TrlduKq4s4_ST_AhulfHgbV-Qf5cXf8-vm9vfVzfnZ7eNF7otjZedM9gB77hiOggPul71elA4KG-E0INpgfm2l062rfDeODDBqH4AKbUQC3Ky6btO8XHGXOy4zB5XdRCMc7Zc8g4UtFXMghx_QR_inKY6XaWMYUYr3lWKbyifYs4Jg12n5ejSs2Vg__u2G9-2-ravvi2roaO31nM_4vAReRdcAbEBcn2a7jB9_v1N2xcpvYod</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>da Silva, Léa Assed Bezerra</creator><creator>Longo, Daniele Lucca</creator><creator>Stuani, Maria Bernadete Sasso</creator><creator>de Queiroz, Alexandra Mussolino</creator><creator>da Silva, Raquel Assed Bezerra</creator><creator>Nelson-Filho, Paulo</creator><creator>Vieira, Heloisa Aparecida Orsini</creator><creator>Pucinelli, Carolina Maschietto</creator><creator>Paula-Silva, Francisco Wanderley Garcia</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0230-1347</orcidid></search><sort><creationdate>20210301</creationdate><title>Effect of root surface treatment with denusomab after delayed tooth replantation</title><author>da Silva, Léa Assed Bezerra ; Longo, Daniele Lucca ; Stuani, Maria Bernadete Sasso ; de Queiroz, Alexandra Mussolino ; da Silva, Raquel Assed Bezerra ; Nelson-Filho, Paulo ; Vieira, Heloisa Aparecida Orsini ; Pucinelli, Carolina Maschietto ; Paula-Silva, Francisco Wanderley Garcia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-c49a8e90292617f3c07c49b7d6ed6c8337d8501c5b4a4553cc8a08f86bd044733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Ankylosis</topic><topic>Bone turnover</topic><topic>Calcium hydroxide</topic><topic>Contamination</topic><topic>Dental roots</topic><topic>Dentistry</topic><topic>Immunohistochemistry</topic><topic>Immunotherapy</topic><topic>Incisor</topic><topic>Incisors</topic><topic>Labeling</topic><topic>Medicine</topic><topic>Monoclonal antibodies</topic><topic>Original Article</topic><topic>Osteoprotegerin</topic><topic>Periodontal Ligament</topic><topic>Rats</topic><topic>Root canals</topic><topic>Root resorption</topic><topic>Root Resorption - prevention & control</topic><topic>Teeth</topic><topic>Tooth Ankylosis</topic><topic>Tooth Replantation</topic><topic>Tooth Root</topic><topic>TRANCE protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>da Silva, Léa Assed Bezerra</creatorcontrib><creatorcontrib>Longo, Daniele Lucca</creatorcontrib><creatorcontrib>Stuani, Maria Bernadete Sasso</creatorcontrib><creatorcontrib>de Queiroz, Alexandra Mussolino</creatorcontrib><creatorcontrib>da Silva, Raquel Assed Bezerra</creatorcontrib><creatorcontrib>Nelson-Filho, Paulo</creatorcontrib><creatorcontrib>Vieira, Heloisa Aparecida Orsini</creatorcontrib><creatorcontrib>Pucinelli, Carolina Maschietto</creatorcontrib><creatorcontrib>Paula-Silva, Francisco Wanderley Garcia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>da Silva, Léa Assed Bezerra</au><au>Longo, Daniele Lucca</au><au>Stuani, Maria Bernadete Sasso</au><au>de Queiroz, Alexandra Mussolino</au><au>da Silva, Raquel Assed Bezerra</au><au>Nelson-Filho, Paulo</au><au>Vieira, Heloisa Aparecida Orsini</au><au>Pucinelli, Carolina Maschietto</au><au>Paula-Silva, Francisco Wanderley Garcia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of root surface treatment with denusomab after delayed tooth replantation</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2021-03-01</date><risdate>2021</risdate><volume>25</volume><issue>3</issue><spage>1255</spage><epage>1264</epage><pages>1255-1264</pages><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objectives
To evaluate denosumab, a human monoclonal antibody that mimics the effects of osteoprotegerin in bone metabolism, as a topical treatment of root surface to be used prior to delayed tooth replantation.
Materials and methods
Thirty-six rats’ right incisors were used. Teeth were extracted and divided into: delayed replantation without root surface treatment (control); delayed replantation with root surface treatment with denosumab 60 mg/mL and 30 mg/mL, respectively, for 10 min both experimentals groups. After that, the root canals were filled with calcium hydroxide and replanted. After 15 and 60 days, the animals were euthanized, and the samples were collected and processed for microscopic analysis. Histological sections were performed, and stained with HE to describe the dental characteristics, measure ankylosis, replacement resorption, and dental resorption by conventional microscopy. Also, was performed Brown & Brenn staining and immunohistochemistry for RANKL, OPG, and periostin.
Results
Denosumab 60 mg/mL reducted ankylosis (
p
< 0.0001), replacement resorption (
p
< 0.0001), and tooth resorption, 60 days after replantation, compared to untreated replanted teeth (
p
< 0.005). Lower bacterial contamination in root surface in the denosumab treatment groups was found, regardless of the concentration used (
p
< 0.001). Also, denosumab treatment inhibited the expression of RANKL without modulating OPG. Periostin was observed in periodontal ligament of replanted tooth, although this labelling was absent in the ankylosis areas, in both experimental periods.
Conclusion
Treatment of the root surface with denosumab at 60 mg/mL of rat teeth before delayed replantation reduced dental root resorption compared with the untreated teeth after 60 days.
Clinical relevance
Survival of a replanted tooth has been a challenge in clinical practice. The use of a medication, such as denosumab, to limit dental root resorption represents an important therapeutical approach.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32728940</pmid><doi>10.1007/s00784-020-03430-1</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-0230-1347</orcidid></addata></record> |
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| ispartof | Clinical oral investigations, 2021-03, Vol.25 (3), p.1255-1264 |
| issn | 1432-6981 1436-3771 |
| language | eng |
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| source | Springer Nature |
| subjects | Animals Ankylosis Bone turnover Calcium hydroxide Contamination Dental roots Dentistry Immunohistochemistry Immunotherapy Incisor Incisors Labeling Medicine Monoclonal antibodies Original Article Osteoprotegerin Periodontal Ligament Rats Root canals Root resorption Root Resorption - prevention & control Teeth Tooth Ankylosis Tooth Replantation Tooth Root TRANCE protein |
| title | Effect of root surface treatment with denusomab after delayed tooth replantation |
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