Enhancement of PCR efficiency using mono- and disaccharides

Polymerase chain reaction is the most commonly used approach for nucleic acids amplification. Despite the variety of PCR methods have been proposed, new techniques are being developed to improve this reaction. We found that, in general, mono- and disaccharides can serve as effective PCR enhancers. U...

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Bibliographic Details
Published in:Analytical biochemistry 2020-10, Vol.606, p.113858-113858, Article 113858
Main Authors: Sakhabutdinova, Assol R., Chemeris, Alexey V., Garafutdinov, Ravil R.
Format: Article
Language:English
Subjects:
Animals
Carbohydrates
Carbohydrates - chemistry
Disaccharides - chemistry
DNA - analysis
Genome, Human
Genome, Insect
Genome, Viral
Humans
Monosaccharides - chemistry
PCR enhancers
Polymerase chain reaction (PCR)
Polymerase Chain Reaction - methods
Sucrose
Trehalose
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Summary:Polymerase chain reaction is the most commonly used approach for nucleic acids amplification. Despite the variety of PCR methods have been proposed, new techniques are being developed to improve this reaction. We found that, in general, mono- and disaccharides can serve as effective PCR enhancers. Unlike oligo- and polysaccharides, low molecular-weight carbohydrates accelerate amplification and increase products yield. The ability of carbohydrates to enhance PCR is not related to their reducing property. The best result was obtained for sucrose providing the most specific and reliable amplification. The effect of carbohydrates is leveled as the size of the amplification region increases. [Display omitted] •Mono- and disaccharides significantly accelerate polymerase chain reaction.•Sucrose provides the most specific, reliable and productive PCR amplification.•Oligo- and polysaccharides do not able to improve polymerase chain reaction.•The beneficial influence of carbohydrates is leveled for long DNA targets.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2020.113858