Ubiquitin-conjugating enzyme E2T(UBE2T) promotes colorectal cancer progression by facilitating ubiquitination and degradation of p53

•UBE2T expression was up-regulated in CRC tissues and cell lines.•Effect of UBE2T on CRC cell line growth and proliferation.•UBE2T regulate the cell migration and invasion in CRC.•UBE2T promotes the progression of CRC by ubiquitination of p53.•Knockdown of UBE2T inhibited the growth of transplanted...

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Published in:Clinics and research in hepatology and gastroenterology 2021-03, Vol.45 (2), p.101493-101493, Article 101493
Main Authors: Wu, Mengqiong, Li, Xianglu, Huang, Weiwei, Chen, Yiming, Wang, Baochun, Liu, Xin
Format: Article
Language:English
Subjects:
Animals
Cell Line, Tumor
Cell Proliferation
Colorectal Neoplasms - genetics
CRC
E-cadherin
Gene Expression Regulation, Neoplastic
Humans
Mice
p53
Tumor Suppressor Protein p53 - genetics
Tumor Suppressor Protein p53 - metabolism
UBE2T
Ubiquitin-Conjugating Enzymes - genetics
Ubiquitin-Conjugating Enzymes - metabolism
Ubiquitination
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Summary:•UBE2T expression was up-regulated in CRC tissues and cell lines.•Effect of UBE2T on CRC cell line growth and proliferation.•UBE2T regulate the cell migration and invasion in CRC.•UBE2T promotes the progression of CRC by ubiquitination of p53.•Knockdown of UBE2T inhibited the growth of transplanted tumor in mice. The expression level of Ubiquitin-conjugating enzyme E2T (UBE2T) is upregulated in various types of human tumors. We explored the correlation and regulatory mechanism of UBE2T in the development of colorectal cancer (CRC). Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to examine the expression of UBE2T in the CRC tissues and cell lines. Immunohistochemical staining, spearman correlation analysis, and Kaplan Meier-survival analysis were used to demonstrate the correlation between UBE2T high expression level and the clinical characteristics of malignant patients and the overall survival. The proliferation, apoptosis, migration and invasion of CRC cells were analyzed by cell transfection, MTT, colony formation, scratch assay, transwell, and flow cytometry. Furthermore, the expression of cell proliferation and apoptosis related proteins and ubiquitination of p53 were detected by western blot. UBE2T was up-regulated in CRC tissues and cell lines, and high expression level of UBE2T was correlated with the clinical characteristics of malignant of CRC patients (P
ISSN:2210-7401
2210-741X
DOI:10.1016/j.clinre.2020.06.018