Process optimization of fucoxanthin production with Tisochrysis lutea

• Published in: Bioresource technology 2020-11, Vol.315, p.123894-123894, Article 123894
• Main Authors: Gao, Fengzheng, Teles (Cabanelas, ITD), Iago, Wijffels, René H, Barbosa, Maria J
• Format: Article
• Language: English
• Subjects: Absorbed light; Dilution rate; Fucoxanthin; Temperature; Tisochrysis lutea
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2020.123894

Process optimization of fucoxanthin production. [Display omitted] •Highest ever reported fucoxanthin (Fx) productivity in Tisochrysis (9.81 mg L−1 d−1).•Optimum light at 300 μmol m−2 s−1 and temperature at 30 °C for Fx productivity.•Fx content (3.68–16.39 mg/g) increases with decreasing daily light absorbed.•High dilution rate (0.53, 0.80 d−1) leads to higher Fx productivity. To optimize fucoxanthin production in Tisochrysis lutea, the effect of different process parameters on fucoxanthin productivity (Pfx) were evaluated using batch and continuous experiments. In batch, the highest Pfx was found at 30 °C and 300 μmol m−2 s−1, allowing to design continuous experiments to optimize the dilution rate. The highest ever reported Pfx (9.43–9.81 mg L−1 d−1) was achieved at dilution rates of 0.53 and 0.80 d−1. Irradiance was varied (50–500 μmol m−2 s−1) to result in a range of absorbed light between 2.23 and 25.80 mol m−2 d−1 at a fixed dilution rate (0.53 d−1). These experiments validated the hypothesis that light absorbed can be used to predict fucoxanthin content, resulting in 2.23 mol m−2 d−1 triggering the highest fucoxanthin content (16.39 mg/g). The highest Pfx was found with 18.38 mol m−2 d−1. These results can be used to achieve high Pfx or fucoxanthin content during cultivation of Tisochrysis lutea.