Enhanced exopolysaccharide production in submerged fermentation of Ganoderma lucidum by Tween 80 supplementation

Bioactive polysaccharides extracted from Ganoderma lucidum ( G. lucidum ) have been widely applied in food and medicine for their multiple functions. In this study, G. lucidum exopolysaccharide (EPS) production in submerged fermentation was stimulated by Tween 80. The addition of 0.25% Tween 80 on d...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2021, Vol.44 (1), p.47-56
Main Authors: Yang, Xiaobing, Yang, Yingyin, Zhang, Yifan, He, Jiahao, Xie, Yizhen
Format: Article
Language:English
Subjects:
Antioxidants
Biological activity
Biosynthesis
Biotechnology
Carbohydrates
Cell membranes
Cell walls
Chemistry
Chemistry and Materials Science
Dietary supplements
Environmental Engineering/Biotechnology
Exopolysaccharides
Fermentation
Food Science
Ganoderma lucidum
Gene expression
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Membrane permeability
Molecular weight
Mushrooms
Mycelia
Polysaccharides
Reactive oxygen species
Research Paper
Saccharides
Uronic acid
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Summary:Bioactive polysaccharides extracted from Ganoderma lucidum ( G. lucidum ) have been widely applied in food and medicine for their multiple functions. In this study, G. lucidum exopolysaccharide (EPS) production in submerged fermentation was stimulated by Tween 80. The addition of 0.25% Tween 80 on day 3 gave a maximum production of mycelial biomass and EPS, with an increase of 19.76 and 137.50%, respectively. Analysis of fermentation kinetics showed that glucose was consumed faster after adding Tween 80, while the expression of EPS biosynthesis-related genes and ATP generation were greatly improved. Moreover, Tween 80 resulted in the significant accumulation of reactive oxygen species and increased cell membrane and cell wall permeability. The EPS from Tween 80-containing medium had higher contents of carbohydrate and uronic acid, lower molecular weight, and higher antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals than those of EPS produced in the absence of Tween 80. This study provides further evidence to clarify the stimulatory effects of Tween 80 in fermentation and provides a guide for the production of bioactive G. lucidum EPS.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-020-02418-1